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. 1981 Jun;78(6):3867–3871. doi: 10.1073/pnas.78.6.3867

Gustin concentration changes relative to salivary zinc and taste in humans.

A R Shatzman, R I Henkin
PMCID: PMC319674  PMID: 6943587

Abstract

Biochemical characteristics of gustin, the major zinc protein in human parotid saliva, are similar whether the protein is isolated from subjects with normal taste acuity or from patients with hypogeusia (who may have a little as 1/5th as much parotid saliva gustin as normal subjects do). Zinc concentration in fraction II of parotid saliva, the fraction in which gustin is found on Sephadex G-150 or Sephacryl S-200 column chromatography, is proportional to the gustin content of saliva and is decreased in patients with lower than normal total parotid saliva zinc. The quantity and spectrophotometric indices of all other protein fractions isolated from patients by these column chromatographic techniques did not differ from those of normals. One patient with proven hypogeusia and low concentrations of zinc in total parotid saliva and fraction II, after 9 days of treatment with exogenous zinc, showed a 150% increase in fraction II zinc and a concomitant increase in apparent gustin levels; these changes preceded the return of normal taste function. These data demonstrate that zinc treatment can affect both taste and gustin concentrations in hypogeusia.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Antoniou L. D., Shalhoub R. J., Sudhakar T., Smith J. C., Jr Reversal of uraemic impotence by zinc. Lancet. 1977 Oct 29;2(8044):895–898. doi: 10.1016/s0140-6736(77)90832-7. [DOI] [PubMed] [Google Scholar]
  2. Arneberg P. Partial characterization of five glycoprotein fractions secreted by the human parotid glands. Arch Oral Biol. 1974 Oct;19(10):921–928. doi: 10.1016/0003-9969(74)90055-7. [DOI] [PubMed] [Google Scholar]
  3. Atkin-Thor E., Goddard B. W., O'Nion J., Stephen R. L., Kolff W. J. Hypogeusia and zinc depletion in chronic dialysis patients. Am J Clin Nutr. 1978 Oct;31(10):1948–1951. doi: 10.1093/ajcn/31.10.1948. [DOI] [PubMed] [Google Scholar]
  4. Beidler L. M., Smallman R. L. Renewal of cells within taste buds. J Cell Biol. 1965 Nov;27(2):263–272. doi: 10.1083/jcb.27.2.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bennick A. Chemical and physical characterization of a phosphoprotein, Protein C, from human saliva and comparison with a related protein A. Biochem J. 1977 May 1;163(2):229–239. doi: 10.1042/bj1630229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bennick A., Connell G. E. Purification and partial characterization of four proteins from human parotid saliva. Biochem J. 1971 Jul;123(3):455–464. doi: 10.1042/bj1230455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bremner I., Davies N. T. The induction of metallothionein in rat liver by zinc injection and restriction of food intake. Biochem J. 1975 Sep;149(3):733–738. doi: 10.1042/bj1490733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Bremner I., Young B. W. Isolation of (copper, zinc-) thioneins from pig liver. Biochem J. 1976 Jun 1;155(3):631–635. doi: 10.1042/bj1550631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hambidge K. M., Hambidge C., Jacobs M., Baum J. D. Low levels of zinc in hair, anorexia, poor growth, and hypogeusia in children. Pediatr Res. 1972 Dec;6(12):868–874. doi: 10.1203/00006450-197212000-00003. [DOI] [PubMed] [Google Scholar]
  10. Hay D. I., Oppenheim F. G. The isolation from human parotid saliva of a further group of proline-rich proteins. Arch Oral Biol. 1974 Aug;19(8):627–632. doi: 10.1016/0003-9969(74)90130-7. [DOI] [PubMed] [Google Scholar]
  11. Henkin R. I., Lippoldt R. E., Bilstad J., Edelhoch H. A zinc protein isolated from human parotid saliva. Proc Natl Acad Sci U S A. 1975 Feb;72(2):488–492. doi: 10.1073/pnas.72.2.488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Henkin R. I., Lippoldt R. E., Bilstad J., Wolf R. O., Lum C. K., Edelhoch H. Fractionation of human parotid saliva proteins. J Biol Chem. 1978 Oct 25;253(20):7556–7565. [PubMed] [Google Scholar]
  13. Henkin R. I., Mueller C. W., Wolf R. O. Estimation of zinc concentration of parotid saliva by flameless atomic absorption spectrophotometry in normal subjects and in patients with idiopathic hypogeusia. J Lab Clin Med. 1975 Jul;86(1):175–180. [PubMed] [Google Scholar]
  14. Henkin R. I., Schechter P. J., Hoye R., Mattern C. F. Idiopathic hypogeusia with dysgeusia, hyposmia, and dysosmia. A new syndrome. JAMA. 1971 Jul 26;217(4):434–440. [PubMed] [Google Scholar]
  15. Henkin R. I., Schecter P. J., Friedewald W. T., Demets D. L., Raff M. A double blind study of the effects of zinc sulfate on taste and smell dysfunction. Am J Med Sci. 1976 Nov-Dec;272(3):285–299. doi: 10.1097/00000441-197611000-00006. [DOI] [PubMed] [Google Scholar]
  16. KAGI J. H., VALLEE B. L. The role of zinc in alcohol dehydrogenase. V. The effect of metal-binding agents on thestructure of the yeast alcohol dehydrogenase molecule. J Biol Chem. 1960 Nov;235:3188–3192. [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. Lim V. S., Fang V. S. Gonadal dysfunction in uremic men. A study of the hypothalamo-pituitary-testicular axis before and after renal transplantation. Am J Med. 1975 May;58(5):655–662. doi: 10.1016/0002-9343(75)90501-x. [DOI] [PubMed] [Google Scholar]
  19. Mahajan S. K., Prasad A. S., Lambujon J., Abbasi A. A., Briggs W. A., McDonald F. D. Improvement of uremic hypogeusia by zinc: a double-blind study. Am J Clin Nutr. 1980 Jul;33(7):1517–1521. doi: 10.1093/ajcn/33.7.1517. [DOI] [PubMed] [Google Scholar]
  20. Mahajan S. K., Prasad A. S., Rabbani P., Briggs W. A., McDonald F. D. Zinc metabolism in uremia. J Lab Clin Med. 1979 Nov;94(5):693–698. [PubMed] [Google Scholar]
  21. Mansouri K., Halsted J. A., Gombos E. A. Zinc, copper, magnesium and calcium in dialyzed and nondialyzed uremic patients. Arch Intern Med. 1970 Jan;125(1):88–93. [PubMed] [Google Scholar]
  22. Mueller G. P., Chen H. T., Dibbet J. A., Chen H. J., Meites J. Effects of warm and cold temperatures on release of TSH, GH, and prolactin in rats. Proc Soc Exp Biol Med. 1974 Dec;147(3):698–700. doi: 10.3181/00379727-147-38420. [DOI] [PubMed] [Google Scholar]
  23. Oppenheim F. G., Hay D. I., Franzblau C. Proline-rich proteins from human parotid saliva. I. Isolation and partial characterization. Biochemistry. 1971 Nov;10(23):4233–4238. doi: 10.1021/bi00799a013. [DOI] [PubMed] [Google Scholar]
  24. Richards M. P., Cousins R. J. Isolation of an intestinal metallothionein induced by parenteral zinc. Biochem Biophys Res Commun. 1977 Mar 21;75(2):286–294. doi: 10.1016/0006-291x(77)91041-5. [DOI] [PubMed] [Google Scholar]
  25. Rupp H., Weser U. Conversion of metallothionein into Cu-thionein, the possible low molecular weight form of neonatal hepatic mitochondrocuprein. FEBS Lett. 1974 Aug 30;44(3):293–297. doi: 10.1016/0014-5793(74)81161-0. [DOI] [PubMed] [Google Scholar]
  26. Rydén L., Deutsch H. F. Preparation and properties of the major copper-binding component in human fetal liver. Its identification as metallothionein. J Biol Chem. 1978 Jan 25;253(2):519–524. [PubMed] [Google Scholar]
  27. Shaikh Z. A., Smith J. C. The biosynthesis of metallothionein rat liver and kidney after administration of cadmium. Chem Biol Interact. 1976 Dec;15(4):327–336. doi: 10.1016/0009-2797(76)90138-1. [DOI] [PubMed] [Google Scholar]
  28. Shatzman A. R., Henkin R. I. Metal-binding characteristics of the parotid salivary protein gustin. Biochim Biophys Acta. 1980 May 29;623(1):107–118. doi: 10.1016/0005-2795(80)90013-6. [DOI] [PubMed] [Google Scholar]
  29. Solomons N. W., Rosenberg I. H., Sandstead H. H., Vo-Khactu K. P. Zinc deficiency in Crohn's disease. Digestion. 1977;16(1-2):87–95. doi: 10.1159/000198059. [DOI] [PubMed] [Google Scholar]
  30. WADDELL W. J. A simple ultraviolet spectrophotometric method for the determination of protein. J Lab Clin Med. 1956 Aug;48(2):311–314. [PubMed] [Google Scholar]
  31. Zähringer J., Baliga B. S., Munro H. N. Novel mechanism for translational control in regulation of ferritin synthesis by iron. Proc Natl Acad Sci U S A. 1976 Mar;73(3):857–861. doi: 10.1073/pnas.73.3.857. [DOI] [PMC free article] [PubMed] [Google Scholar]

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