Fig. 5.

Effect of various steroid hormones on NPPC-mediated meiotic arrest in vitro. The proportion of oocytes having undergone GVB was determined at 24 h culture with or without (C) various steroid hormones. Bars indicated the mean ± sem of four independent experiments with at least 25 COCs assessed per group in each experiment. All steroid hormones were used at a concentration of 100 nm. Left panel, No NPPC; right panel, 10 nm NPPC; E2, estradiol; T, testosterone; P4, progesterone. Percentages not connected by the same letter are significantly different (P < 0.05).