Abstract
Cultured human lymphoblasts (CCRF-CEM line) have DNA glycosylase activities, the specificities of which were investigated by using high-performance liquid chromatography. In addition to 3-methyladenine, 3-ethyladenine, 7-methylguanine, 7-ethylguanine, 3-methylguanine, and 3-ethylguanine also were excised from alkylated double-stranded DNA and deoxypolynucleotides, but 1-methyladenine, 1-ethyladenine, O6-methylguanine, and O6-ethylguanine were not. The glycosylase activity was generally greater for the methylated than for the corresponding ethylated purines and was also greater toward 3-alkyladenine than toward 3-alkyladenine than toward 3-alkylguanine. 7-Methylguanine and 7-ethylguanine were excised to similar but low extents. However, in molar terms, the release of 7-methylguanine was similar to that of 3-methyladenine.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bodell W. J., Singer B., Thomas G. H., Cleaver J. E. Evidence for removal at different rates of O-ethyl pyrimidines and ethylphosphotriesters in two human fibroblast cell lines. Nucleic Acids Res. 1979 Jun 25;6(8):2819–2829. doi: 10.1093/nar/6.8.2819. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brent T. P. 3-Methyladenine-DNA glycosylase: a probe for determining alkylation damage and repair in human fibroblasts. Biochem Biophys Res Commun. 1979 Dec 14;91(3):795–802. doi: 10.1016/0006-291x(79)91950-8. [DOI] [PubMed] [Google Scholar]
- Brent T. P. Partial purification and characterization of a human 3-methyladenine-DNA glycosylase. Biochemistry. 1979 Mar 6;18(5):911–916. doi: 10.1021/bi00572a028. [DOI] [PubMed] [Google Scholar]
- Goth-Goldstein R. Repair of DNA damaged by alkylating carcinogens is defective in xeroderma pigmentosum-derived fibroblasts. Nature. 1977 May 5;267(5606):81–82. doi: 10.1038/267081a0. [DOI] [PubMed] [Google Scholar]
- Goth R., Rajewsky M. F. Molecular and cellular mechanisms associated with pulse-carcinogenesis in the rat nerbous system by ethyinitrosourea: ethylation of nucleic acids and elimination rates of ethylated bases from the DNA of different tissues. Z Krebsforsch Klin Onkol Cancer Res Clin Oncol. 1974;82(1):37–64. doi: 10.1007/BF00304382. [DOI] [PubMed] [Google Scholar]
- Ishiwata K., Oikawa A. Actions of human DNA glycosylases on uracil-containing DNA, methylated DNA and their reconstituted chromatins. Biochim Biophys Acta. 1979 Jul 26;563(2):375–384. doi: 10.1016/0005-2787(79)90056-x. [DOI] [PubMed] [Google Scholar]
- Karran P., Lindahl T., Griffin B. Adaptive response to alkylating agents involves alteration in situ of O6-methylguanine residues in DNA. Nature. 1979 Jul 5;280(5717):76–77. doi: 10.1038/280076a0. [DOI] [PubMed] [Google Scholar]
- Kirtikar D. M., Goldthwait D. A. The enzymatic release of O6-methylguanine and 3-methyladenine from DNA reacted with the carcinogen N-methyl-N-nitrosourea. Proc Natl Acad Sci U S A. 1974 May;71(5):2022–2026. doi: 10.1073/pnas.71.5.2022. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laval J. Two enzymes are required from strand incision in repair of alkylated DNA. Nature. 1977 Oct 27;269(5631):829–832. doi: 10.1038/269829a0. [DOI] [PubMed] [Google Scholar]
- Lawley P. D., Orr D. J. Specific excision of methylation products from DNA of Escherichia coli treated with N-methyl-N'-nitro-N-nitrosoguanidine. Chem Biol Interact. 1970 Aug;2(2):154–157. doi: 10.1016/0009-2797(70)90047-5. [DOI] [PubMed] [Google Scholar]
- Lawley P. D., Warren W. Removal of minor methylation products 7-methyladenine and 3-methylguanine from DNA of Escherichia coli treated with dimethyl sulphate. Chem Biol Interact. 1976 Feb;12(2):211–220. doi: 10.1016/0009-2797(76)90100-9. [DOI] [PubMed] [Google Scholar]
- Margison G. P., Margison J. M., Montesano R. Methylated purines in the deoxyribonucleic acid of various Syrian-golden-hamster tissues after administration of a hepatocarcinogenic dose of dimethylnitrosamine. Biochem J. 1976 Sep 1;157(3):627–634. doi: 10.1042/bj1570627. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pegg A. E. Enzymatic removal of O6-methylguanine from DNA by mammalian cell extracts. Biochem Biophys Res Commun. 1978 Sep 14;84(1):166–173. doi: 10.1016/0006-291x(78)90278-4. [DOI] [PubMed] [Google Scholar]
- Riazuddin S., Lindahl T. Properties of 3-methyladenine-DNA glycosylase from Escherichia coli. Biochemistry. 1978 May 30;17(11):2110–2118. doi: 10.1021/bi00604a014. [DOI] [PubMed] [Google Scholar]
- Shackleton J., Warren W., Roberts J. J. The excision of N-methyl-N-nitrosourea-induced lesions from the DNA of Chinese hamster cells as measured by the loss of sites sensitive to an enzyme extract that excises 3-methylpurines but not O6-methylguanine. Eur J Biochem. 1979 Jul;97(2):425–433. doi: 10.1111/j.1432-1033.1979.tb13130.x. [DOI] [PubMed] [Google Scholar]
- Singer B. All oxygens in nucleic acids react with carcinogenic ethylating agents. Nature. 1976 Nov 25;264(5584):333–339. doi: 10.1038/264333a0. [DOI] [PubMed] [Google Scholar]
- Singer B., Bodell W. J., Cleaver J. E., Thomas G. H., Rajewsky M. F., Thon W. Oxygens in DNA are main targets for ethylnitrosourea in normal and xeroderma pigmentosum fibroblasts and fetal rat brain cells. Nature. 1978 Nov 2;276(5683):85–88. doi: 10.1038/276085a0. [DOI] [PubMed] [Google Scholar]
- Singer B., Kröger M., Carrano M. O2- and O4-alkyl pyrimidine nucleosides: stability of the glycosyl bond and of the alkyl group as a function of pH. Biochemistry. 1978 Apr 4;17(7):1246–1250. doi: 10.1021/bi00600a018. [DOI] [PubMed] [Google Scholar]
- Singer B. N-nitroso alkylating agents: formation and persistence of alkyl derivatives in mammalian nucleic acids as contributing factors in carcinogenesis. J Natl Cancer Inst. 1979 Jun;62(6):1329–1339. [PubMed] [Google Scholar]
- Singer B. The chemical effects of nucleic acid alkylation and their relation to mutagenesis and carcinogenesis. Prog Nucleic Acid Res Mol Biol. 1975;15(0):219–284. [PubMed] [Google Scholar]
- Steward A. P., Scherer E., Emmelot P. Formation of relatively persistent O2-ethylthymidine by diethylnitrosamine in rat liver DNA. FEBS Lett. 1979 Apr 1;100(1):191–194. doi: 10.1016/0014-5793(79)81161-8. [DOI] [PubMed] [Google Scholar]
- Warren W., Crathorn A. R., Shooter K. V. The stability of methylated purines and of methylphosphotriesters in the DNA of V79 cells after treatment with N-methyl-N-nitrosourea. Biochim Biophys Acta. 1979 Jun 20;563(1):82–88. doi: 10.1016/0005-2787(79)90009-1. [DOI] [PubMed] [Google Scholar]