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. 1981 Feb;78(2):981–985. doi: 10.1073/pnas.78.2.981

Photoaffinity labeling of an herbicide receptor protein in chloroplast membranes

Klaus Pfister 1,*, Katherine E Steinback 1,†, Gary Gardner 1,‡, Charles J Arntzen 1,†
PMCID: PMC319929  PMID: 16592984

Abstract

2-Azido-4-ethylamino-6-isopropylamino-s-triazine (azido-atrazine) inhibits photosynthetic electron transport at a site identical to that affected by atrazine (2-chloro-4-ethylamino-6-isopropylamino-s-triazine). The latter is a well-characterized inhibitor of photosystem II reactions. Azido-atrazine was used as a photoaffinity label to identify the herbicide receptor protein; UV irradiation of chloroplast thylakoids in the presence of azido[14C]atrazine resulted in the covalent attachment of radioactive inhibitor to thylakoid membranes isolated from pea seedlings and from a triazine-susceptible biotype of the weed Amaranthus hybridus. No covalent binding of azido-atrazine was observed for thylakoid membranes isolated from a naturally occurring triazine-resistant biotype of A. hybridus. Analysis of thylakoid polypeptides from both the susceptible and resistant A. hybridus biotypes by sodium dodecyl sulfate/polyacrylamide gel electrophoresis, followed by fluorography to locate 14C label, demonstrated specific association of the azido[14C]atrazine with polypeptides of the 34- to 32-kilodalton size class in susceptible but not in resistant membranes.

Keywords: triazines, photosystem II, electron transport inhibitors, diuron

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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