Skip to main content
. Author manuscript; available in PMC: 2011 Oct 24.
Published in final edited form as: Biochemistry. 2006 Jul 25;45(29):8978–8987. doi: 10.1021/bi060440o

Figure 8. Mass spectrometry of GRx1, sporidesmin and reaction products.

Figure 8

MALDI spectra of GRx1 and the reaction product with sporidesmin show ions at m/z 11,645 corresponding to native GRx1 (A), and m/z 12,054 consistent with formation of a mixed disulfide between GRx1 and sporidesmin followed by elimination of two sulfur atoms (B). ESI spectra of sporidesmin (C) and the corresponding low mass regions in the product of reaction with GRx1 (D) contain ions consistent with the presence of sporidesmin (calculated m/z 474.0555), sporidesmin minus two sulfur atoms (calculated mass 410.1113) and in the reaction product ions corresponding to sporidesmin in which a sulfur has been replaced by hydrogen (calculated m/z 443.0912) and the product of monooxygenation of the remaining sulfur atom (calculated m/z 459.0862). The identity of the m/z 479 species is unknown. Low mass regions of the spectra were internally calibrated using the sporidesmin ion.