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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1981 Feb;78(2):1162–1166. doi: 10.1073/pnas.78.2.1162

Complete amino acid sequence of bovine thymosin beta 4: a thymic hormone that induces terminal deoxynucleotidyl transferase activity in thymocyte populations.

T L Low, S K Hu, A L Goldstein
PMCID: PMC319967  PMID: 6940133

Abstract

The amino acid sequence of thymosin beta 4, a polypeptide isolated from calf thymus, was determined. Thymosin beta 4 is composed of 43 amino acid residues and has a molecular weight of 4982 and an isoelectric point of 5.1. The NH2 terminus of the peptide is blocked by an acetyl group. This molecule induces expression of terminal deoxynucleotidyl transferase (DNA nucleotidylexotransferase, EC 2.7.7.31) in transferase-negative murine thymocytes in vivo and in citro. Thus, it appears that thymosin beta 4 acts on lymphoid stem cells and may control the early stages of the maturation process of thymus-dependent lymphocytes. This peptide is one of several present in thymosin fraction 5 that participates in the regulation, differentiation, and function of thymus-dependent thymocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahmed A., Wong D. M., Thurman G. B., Low T. L., Goldstein A. L., Sharkis S. J., Goldschneider I. T-lymphocyte maturation: cell surface markers and immune function induced by T-lymphocyte cell-free products and thymosin polypeptides. Ann N Y Acad Sci. 1979;332:81–94. doi: 10.1111/j.1749-6632.1979.tb47100.x. [DOI] [PubMed] [Google Scholar]
  2. Bach J. F., Dardenne M., Goldstein A. L., Guha A., White A. Appearance of T-cell markers in bone marrow rosette-forming cells after incubation with thymosin, a thymic hormone. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2734–2738. doi: 10.1073/pnas.68.11.2734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barton R., Goldschneider I., Bollum F. J. The distribution of terminal deoxynucleotidyl transferase (TdT) among subsets of thymocytes in the rat. J Immunol. 1976 Feb;116(2):462–468. [PubMed] [Google Scholar]
  4. Chang L. M. Development of terminal deoxynucleotidyl transferase activity in embryonic calf thymus gland. Biochem Biophys Res Commun. 1971 Jul 2;44(1):124–131. doi: 10.1016/s0006-291x(71)80167-5. [DOI] [PubMed] [Google Scholar]
  5. Coleman M. S., Hutton J. J., Bollum F. J. Terminal deoxynucleotidyl transferase and DNA polymerase in classes of cells from rat thymus. Biochem Biophys Res Commun. 1974 Jun 18;58(4):1104–1109. doi: 10.1016/s0006-291x(74)80257-3. [DOI] [PubMed] [Google Scholar]
  6. Dauphinee M. J., Talal N., Goldstein A. L., White A. Thymosin corrects the abnormal DNA synthetic response of NZB mouse thymocytes. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2637–2641. doi: 10.1073/pnas.71.7.2637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fish W. W., Mann K. G., Tanford C. The estimation of polypeptide chain molecular weights by gel filtration in 6 M guanidine hydrochloride. J Biol Chem. 1969 Sep 25;244(18):4989–4994. [PubMed] [Google Scholar]
  8. Goldstein A. L., Cohen G. H., Rossio J. L., Thurman G. B., Brown C. N., Ulrich J. T. Use of thymosin in the treatment of primary immunodeficiency diseases and cancer. Med Clin North Am. 1976 May;60(3):591–606. doi: 10.1016/s0025-7125(16)31900-9. [DOI] [PubMed] [Google Scholar]
  9. Goldstein A. L., Low T. L., McAdoo M., McClure J., Thurman G. B., Rossio J., Lai C. Y., Chang D., Wang S. S., Harvey C. Thymosin alpha1: isolation and sequence analysis of an immunologically active thymic polypeptide. Proc Natl Acad Sci U S A. 1977 Feb;74(2):725–729. doi: 10.1073/pnas.74.2.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Goldstein A. L., Slater F. D., White A. Preparation, assay, and partial purification of a thymic lymphocytopoietic factor (thymosin). Proc Natl Acad Sci U S A. 1966 Sep;56(3):1010–1017. doi: 10.1073/pnas.56.3.1010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gregoire K. E., Goldschneider I., Barton R. W., Bollum F. J. Intracellular distribution of terminal deoxynucleotidyl transferase in rat bone marrow and thymus. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3993–3996. doi: 10.1073/pnas.74.9.3993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hardy M. A., Zisblatt M., Levine N., Goldstein A. L., Lilly F., White A. Reversal by thymosin of increased susceptibility of immunosuppressed mice to Moloney sarcoma virus. Transplant Proc. 1971 Mar;3(1):926–928. [PubMed] [Google Scholar]
  13. Hooper J. A., McDaniel M. C., Thurman G. B., Cohen G. H., Schulof R. S., Goldstein A. L. Purification and properties of bovine thymosin. Ann N Y Acad Sci. 1975 Feb 28;249:125–144. doi: 10.1111/j.1749-6632.1975.tb29063.x. [DOI] [PubMed] [Google Scholar]
  14. Khaw B. A., Rule A. H. Immunotherapy of the Dunning leukaemia with thymic extracts. Br J Cancer. 1973 Oct;28(4):288–292. doi: 10.1038/bjc.1973.150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kung P. C., Siverstone A. E., McCaffrey R. P., Baltimore D. Murine terminal deoxynucleotidyl transferase: cellular distribution and response to cortisone. J Exp Med. 1975 Apr 1;141(4):855–865. [PMC free article] [PubMed] [Google Scholar]
  16. Low T. L., Goldstein A. L. The chemistry and biology of thymosin. II. Amino acid sequence analysis of thymosin alpha1 and polypeptide beta1. J Biol Chem. 1979 Feb 10;254(3):987–995. [PubMed] [Google Scholar]
  17. Low T. L., Thurman G. B., McAdoo M., McClure J., Rossio J. L., Naylor P. H., Goldstein A. L. The chemistry and biology of thymosin. I. Isolation, characterization, and biological activities of thymosin alpha1 and polypeptide beta1 from calf thymus. J Biol Chem. 1979 Feb 10;254(3):981–986. [PubMed] [Google Scholar]
  18. Mendez E., Lai C. Y. Reaction of peptides with fluorescamine on paper after chromatography or electrophoresis. Anal Biochem. 1975 May 12;65(1-2):281–292. doi: 10.1016/0003-2697(75)90511-4. [DOI] [PubMed] [Google Scholar]
  19. Nakai N., Lai C. Y., Horecker B. L. Use of fluorescamine in the chromatographic analysis of peptides from proteins. Anal Biochem. 1974 Apr;58(2):563–570. doi: 10.1016/0003-2697(74)90225-5. [DOI] [PubMed] [Google Scholar]
  20. Offord R. E. Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups. Nature. 1966 Aug 6;211(5049):591–593. doi: 10.1038/211591a0. [DOI] [PubMed] [Google Scholar]
  21. Pazmiño N. H., Ihle J. N., Goldstein A. L. Induction in vivo and in vitro of terminal deoxynucleotidyl transferase by thymosin in bone marrow cells from athymic mice. J Exp Med. 1978 Mar 1;147(3):708–718. doi: 10.1084/jem.147.3.708. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Pazmiño N. H., Ihle J. N., McEwan R. N., Goldstein A. L. Control of differentiation of thymocyte precursors in the bone marrow by thymic hormones. Cancer Treat Rep. 1978 Nov;62(11):1749–1755. [PubMed] [Google Scholar]
  23. Pazmiño N. H., Ihle J. N. Strain-, age-, and tumor-dependent distribution of terminal deoxynucleotidyl transferase in thymocytes of mice. J Immunol. 1976 Aug;117(2):620–625. [PubMed] [Google Scholar]
  24. Penke B., Ferenczi R., Kovács K. A new acid hydrolysis method for determining tryptophan in peptides and proteins. Anal Biochem. 1974 Jul;60(1):45–50. doi: 10.1016/0003-2697(74)90129-8. [DOI] [PubMed] [Google Scholar]
  25. Schafer L. A., Goldstein A. L., Gutterman J. U., Hersh E. M. In vitro and in vivo studies with thymosin in cancer patients. Ann N Y Acad Sci. 1976;277(00):609–620. doi: 10.1111/j.1749-6632.1976.tb41733.x. [DOI] [PubMed] [Google Scholar]
  26. Scheinberg M. A., Goldstein A. L., Cathcart E. S. Thymosin restores T cell function and reduces the incidence of amyloid disease in casein-treated mice. J Immunol. 1976 Jan;116(1):156–158. [PubMed] [Google Scholar]
  27. Schmer G., Kreil G. Micro method for detection of formyl and acetyl groups in proteins. Anal Biochem. 1969 May;29(2):186–192. doi: 10.1016/0003-2697(69)90301-7. [DOI] [PubMed] [Google Scholar]
  28. Spencer R. L., Wold F. A new convenient method for estimation of total cystine-cysteine in proteins. Anal Biochem. 1969 Oct 15;32(1):185–190. doi: 10.1016/0003-2697(69)90123-7. [DOI] [PubMed] [Google Scholar]
  29. Wara D. W., Ammann A. J. Editorial: Thymic cells and humoral factors as therapeutic agents. Pediatrics. 1976 May;57(5):643–646. [PubMed] [Google Scholar]
  30. Wara D. W., Goldstein A. L., Doyle N. E., Ammann A. J. Thymosin activity in patients with cellular immunodeficiency. N Engl J Med. 1975 Jan 9;292(2):70–74. doi: 10.1056/NEJM197501092920204. [DOI] [PubMed] [Google Scholar]
  31. Zisblatt M., Goldstein A. L., Lilly F., White A. Acceleration by thymosin of the development of resistance to murine sarcoma virus-induced tumor in mice. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1170–1174. doi: 10.1073/pnas.66.4.1170. [DOI] [PMC free article] [PubMed] [Google Scholar]

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