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. 1981 Feb;78(2):1229–1232. doi: 10.1073/pnas.78.2.1229

Effects of chaotropic and antichaotropic agents on elution of poliovirus adsorbed on membrane filters.

S R Farrah, D O Shah, L O Ingram
PMCID: PMC319981  PMID: 6262758

Abstract

The association of poliovirus with membrane filters results from both electrostatic and hydrophobic interactions. At low pH, electrostatic interactions appear to dominate. However, at high pH, hydrophobic interactions appear to dominate with both Millipore and Zeta plus filters. With both filters, viral elution was prevented at high pH by the presence of antichaotropic salts, which strengthen hydrophobic associations. This effect was antagonized by detergents and by chaotropic salts, which weaken hydrophobic associations. Excellent correlation was observed between viral elution, the solubilization of adenine, and the micelle-formation process in the presence of chaotropic and antichaotropic agents. Such simple measurements of the relative ability of solvents to accommodate hydrophobic groups may be of predictive value in designing methods for concentration and purification of viruses. The hypothesis that hydrophobic interactions are the primary force involved in the stabilization of virus attachment at high pH is consistent with observations that cannot be explained by consideration of electrostatic interactions as the major stabilizing factor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dandliker W. B., Alonso R., de Saussure V. A., Kierszenbaum F., Levison S. A., Schapiro H. C. The effect of chaotropic ions on the dissociation of antigen-antibody complexes. Biochemistry. 1967 May;6(5):1460–1467. doi: 10.1021/bi00857a031. [DOI] [PubMed] [Google Scholar]
  2. Davis K. A., Hatefi Y. Resolution and reconstitution of complex II (succinate-ubiquinone reductase) by salts. Arch Biochem Biophys. 1972 Apr;149(2):505–512. doi: 10.1016/0003-9861(72)90350-5. [DOI] [PubMed] [Google Scholar]
  3. Farrah S. R., Bitton G. Elution of poliovirus adsorbed to membrane filters. Appl Environ Microbiol. 1978 Dec;36(6):982–984. doi: 10.1128/aem.36.6.982-984.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Farrah S. R., Bitton G. Low molecular weight substitutes for beef extract as eluents for poliovirus adsorbed to membrane filters. Can J Microbiol. 1979 Sep;25(9):1045–1051. doi: 10.1139/m79-160. [DOI] [PubMed] [Google Scholar]
  5. Goyal S. M., Zerda K. S., Gerba C. P. Concentration of coliphages from large volumes of water and wastewater. Appl Environ Microbiol. 1980 Jan;39(1):85–91. doi: 10.1128/aem.39.1.85-91.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hatefi Y., Hanstein W. G. Destabilization of membranes with chaotropic ions. Methods Enzymol. 1974;31:770–790. doi: 10.1016/0076-6879(74)31080-4. [DOI] [PubMed] [Google Scholar]
  7. Hatefi Y., Hanstein W. G. Solubilization of particulate proteins and nonelectrolytes by chaotropic agents. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1129–1136. doi: 10.1073/pnas.62.4.1129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mandel B. Characterization of type 1 poliovirus by electrophoretic analysis. Virology. 1971 Jun;44(3):554–568. doi: 10.1016/0042-6822(71)90369-2. [DOI] [PubMed] [Google Scholar]
  9. Rock C. O., Cronan J. E., Jr Solubilization, purification, and salt activation of acyl-acyl carrier protein synthetase from Escherichia coli. J Biol Chem. 1979 Aug 10;254(15):7116–7122. [PubMed] [Google Scholar]
  10. Sobsey M. D., Jones B. L. Concentration of poliovirus from tap water using positively charged microporous filters. Appl Environ Microbiol. 1979 Mar;37(3):588–595. doi: 10.1128/aem.37.3.588-595.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wallis C., Melnick J. L. Concentration of enteroviruses on membrane filters. J Virol. 1967 Jun;1(3):472–477. doi: 10.1128/jvi.1.3.472-477.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wallis C., Melnick J. L., Gerba C. P. Concentration of viruses from water by membrane chromatography. Annu Rev Microbiol. 1979;33:413–437. doi: 10.1146/annurev.mi.33.100179.002213. [DOI] [PubMed] [Google Scholar]

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