Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1984 Jul 25;12(14):5665–5684. doi: 10.1093/nar/12.14.5665

A novel soybean mitochondrial transcript resulting from a DNA rearrangement involving the 5S rRNA gene.

P H Morgens, E A Grabau, R F Gesteland
PMCID: PMC320022  PMID: 6540439

Abstract

A tissue culture line of soybean cells (SB-1) is shown to have an unusual arrangement of mitochondrial genes. Fusion of the mitochondrial 5S gene to the distal end of an unidentified gene results in the abundant expression of an 800 nucleotide RNA with the 5S rRNA at its 5' end. Since only the fused 5S rRNA gene is found in this cell line, the functional 5S rRNA must arise by processing of the 800 nucleotide RNA or by intermittent termination of transcription. The 800 nucleotide transcript and its DNA arrangement are not detected at comparable levels in other soybean sources, including the parent plant of the SB-1 tissue culture line. The role of recombination in the origin of this gene fusion and in plant mitochondrial DNA in general is discussed.

Full text

PDF
5665

Images in this article

5668Image
on p.5668
5674Image
on p.5674
5676Image
on p.5676
5676Image
on p.5676
5677Image
on p.5677
5678Image
on p.5678

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alwine J. C., Kemp D. J., Stark G. R. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350–5354. doi: 10.1073/pnas.74.12.5350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berk A. J., Sharp P. A. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977 Nov;12(3):721–732. doi: 10.1016/0092-8674(77)90272-0. [DOI] [PubMed] [Google Scholar]
  4. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4801–4805. doi: 10.1073/pnas.75.10.4801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brutlag D. L., Clayton J., Friedland P., Kedes L. H. SEQ: a nucleotide sequence analysis and recombination system. Nucleic Acids Res. 1982 Jan 11;10(1):279–294. doi: 10.1093/nar/10.1.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chao S., Sederoff R. R., Levings C. S. Partial Sequence Analysis of the 5S to 18S rRNA Gene Region of the Maize Mitochondrial Genome. Plant Physiol. 1983 Jan;71(1):190–193. doi: 10.1104/pp.71.1.190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dale R. M. Sequence homology among different size classes of plant mtDNAs. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4453–4457. doi: 10.1073/pnas.78.7.4453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dale R. M., Wu M., Kiernan M. C. Analysis of four tobacco mitochondrial DNA size classes. Nucleic Acids Res. 1983 Mar 25;11(6):1673–1685. doi: 10.1093/nar/11.6.1673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gamborg O. L., Miller R. A., Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968 Apr;50(1):151–158. doi: 10.1016/0014-4827(68)90403-5. [DOI] [PubMed] [Google Scholar]
  10. Kemble R. J., Mans R. J. Examination of the mitochondrial genome of revertant progeny from S cms maize with cloned S-1 and S-2 hybridization probes. J Mol Appl Genet. 1983;2(2):161–171. [PubMed] [Google Scholar]
  11. Levings C. S., 3rd, Kim B. D., Pring D. R., Conde M. F., Mans R. J., Laughnan J. R., Gabay-Laughnan S. J. Cytoplasmic Reversion of cms-S in Maize: Association with a Transpositional Event. Science. 1980 Aug 29;209(4460):1021–1023. doi: 10.1126/science.209.4460.1021. [DOI] [PubMed] [Google Scholar]
  12. Levings C. S., 3rd The plant mitochondrial genome and its mutants. Cell. 1983 Mar;32(3):659–661. doi: 10.1016/0092-8674(83)90051-x. [DOI] [PubMed] [Google Scholar]
  13. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  15. Messing J., Gronenborn B., Müller-Hill B., Hans Hopschneider P. Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3642–3646. doi: 10.1073/pnas.74.9.3642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schnare M. N., Gray M. W. 3'-Terminal sequence of wheat mitochondrial 18S ribosomal RNA: further evidence of a eubacterial evolutionary origin. Nucleic Acids Res. 1982 Jul 10;10(13):3921–3932. doi: 10.1093/nar/10.13.3921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sederoff R. R., Levings C. S., Timothy D. H., Hu W. W. Evolution of DNA sequence organization in mitochondrial genomes of Zea. Proc Natl Acad Sci U S A. 1981 Oct;78(10):5953–5957. doi: 10.1073/pnas.78.10.5953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  19. Spencer D. F., Bonen L., Gray M. W. Primary sequence of wheat mitochondrial 5S ribosomal ribonucleic acid: functional and evolutionary implications. Biochemistry. 1981 Jul 7;20(14):4022–4029. doi: 10.1021/bi00517a011. [DOI] [PubMed] [Google Scholar]
  20. Synenki R. M., Levings C. S., Shah D. M. Physicochemical characterization of mitochondrial DNA from soybean. Plant Physiol. 1978 Mar;61(3):460–464. doi: 10.1104/pp.61.3.460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wallace D. C. Structure and evolution of organelle genomes. Microbiol Rev. 1982 Jun;46(2):208–240. doi: 10.1128/mr.46.2.208-240.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ward B. L., Anderson R. S., Bendich A. J. The mitochondrial genome is large and variable in a family of plants (cucurbitaceae). Cell. 1981 Sep;25(3):793–803. doi: 10.1016/0092-8674(81)90187-2. [DOI] [PubMed] [Google Scholar]
  23. Weaver R. F., Weissmann C. Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates. Nucleic Acids Res. 1979 Nov 10;7(5):1175–1193. doi: 10.1093/nar/7.5.1175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wieslander L. A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels. Anal Biochem. 1979 Oct 1;98(2):305–309. doi: 10.1016/0003-2697(79)90145-3. [DOI] [PubMed] [Google Scholar]
  25. Wilbur W. J., Lipman D. J. Rapid similarity searches of nucleic acid and protein data banks. Proc Natl Acad Sci U S A. 1983 Feb;80(3):726–730. doi: 10.1073/pnas.80.3.726. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES