Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1984 Aug 10;12(15):6063–6072. doi: 10.1093/nar/12.15.6063

Molecular cloning and chromosome assignment of murine N-ras.

J Ryan, C P Hart, F H Ruddle
PMCID: PMC320057  PMID: 6089108

Abstract

The murine N-ras gene was cloned by screening an EMBL-3 recombinant phage library with a human N-ras specific probe. Hybridization of two separate unique sequence N-ras probes, isolated from the 5' and 3' flanking sequences of the murine gene, to a mouse-Chinese hamster hybrid mapping panel assigns the N-ras locus to mouse chromosome three.

Full text

PDF
6063

Images in this article

6067Image
on p.6067
6067Image
on p.6067
6068Image
on p.6068
6069Image
on p.6069
6070Image
on p.6070

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balmain A., Ramsden M., Bowden G. T., Smith J. Activation of the mouse cellular Harvey-ras gene in chemically induced benign skin papillomas. Nature. 1984 Feb 16;307(5952):658–660. doi: 10.1038/307658a0. [DOI] [PubMed] [Google Scholar]
  2. Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
  3. Chattopadhyay S. K., Chang E. H., Lander M. R., Ellis R. W., Scolnick E. M., Lowy D. R. Amplification and rearrangement of onc genes in mammalian species. Nature. 1982 Mar 25;296(5855):361–363. doi: 10.1038/296361a0. [DOI] [PubMed] [Google Scholar]
  4. Davis M., Malcolm S., Hall A., Marshall C. J. Localisation of the human N-ras oncogene to chromosome 1cen - p21 by in situ hybridisation. EMBO J. 1983;2(12):2281–2283. doi: 10.1002/j.1460-2075.1983.tb01735.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Der C. J., Krontiris T. G., Cooper G. M. Transforming genes of human bladder and lung carcinoma cell lines are homologous to the ras genes of Harvey and Kirsten sarcoma viruses. Proc Natl Acad Sci U S A. 1982 Jun;79(11):3637–3640. doi: 10.1073/pnas.79.11.3637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Eva A., Aaronson S. A. Frequent activation of c-kis as a transforming gene in fibrosarcomas induced by methylcholanthrene. Science. 1983 May 27;220(4600):955–956. doi: 10.1126/science.6302839. [DOI] [PubMed] [Google Scholar]
  7. Eva A., Tronick S. R., Gol R. A., Pierce J. H., Aaronson S. A. Transforming genes of human hematopoietic tumors: frequent detection of ras-related oncogenes whose activation appears to be independent of tumor phenotype. Proc Natl Acad Sci U S A. 1983 Aug;80(16):4926–4930. doi: 10.1073/pnas.80.16.4926. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Feig L. A., Bast R. C., Jr, Knapp R. C., Cooper G. M. Somatic activation of rasK gene in a human ovarian carcinoma. Science. 1984 Feb 17;223(4637):698–701. doi: 10.1126/science.6695178. [DOI] [PubMed] [Google Scholar]
  9. Frischauf A. M., Lehrach H., Poustka A., Murray N. Lambda replacement vectors carrying polylinker sequences. J Mol Biol. 1983 Nov 15;170(4):827–842. doi: 10.1016/s0022-2836(83)80190-9. [DOI] [PubMed] [Google Scholar]
  10. George D. L., Scott A. F., de Martinville B., Francke U. Amplified DNA in Y1 mouse adrenal tumor cells: isolation of cDNAs complementary to an amplified c-Ki-ras gene and localization of homologous sequences to mouse chromosome 6. Nucleic Acids Res. 1984 Mar 26;12(6):2731–2743. doi: 10.1093/nar/12.6.2731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Goldfarb M., Shimizu K., Perucho M., Wigler M. Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cells. Nature. 1982 Apr 1;296(5856):404–409. doi: 10.1038/296404a0. [DOI] [PubMed] [Google Scholar]
  12. Guerrero I., Calzada P., Mayer A., Pellicer A. A molecular approach to leukemogenesis: mouse lymphomas contain an activated c-ras oncogene. Proc Natl Acad Sci U S A. 1984 Jan;81(1):202–205. doi: 10.1073/pnas.81.1.202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hall A., Marshall C. J., Spurr N. K., Weiss R. A. Identification of transforming gene in two human sarcoma cell lines as a new member of the ras gene family located on chromosome 1. Nature. 1983 Jun 2;303(5916):396–400. doi: 10.1038/303396a0. [DOI] [PubMed] [Google Scholar]
  14. Ish-Horowicz D., Burke J. F. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998. doi: 10.1093/nar/9.13.2989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kourides I. A., Barker P. E., Gurr J. A., Pravtcheva D. D., Ruddle F. H. Assignment of the genes for the alpha and beta subunits of thyrotropin to different mouse chromosomes. Proc Natl Acad Sci U S A. 1984 Jan;81(2):517–519. doi: 10.1073/pnas.81.2.517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kozak C. A., Sears J. F., Hoggan M. D. Genetic mapping of the mouse oncogenes c-Ha-ras-1 and c-fes to chromosome 7. J Virol. 1983 Jul;47(1):217–220. doi: 10.1128/jvi.47.1.217-220.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Murray M. J., Cunningham J. M., Parada L. F., Dautry F., Lebowitz P., Weinberg R. A. The HL-60 transforming sequence: a ras oncogene coexisting with altered myc genes in hematopoietic tumors. Cell. 1983 Jul;33(3):749–757. doi: 10.1016/0092-8674(83)90017-x. [DOI] [PubMed] [Google Scholar]
  18. O'Brien S. J., Nash W. G. Genetic mapping in mammals: chromosome map of domestic cat. Science. 1982 Apr 16;216(4543):257–265. doi: 10.1126/science.7063884. [DOI] [PubMed] [Google Scholar]
  19. Parada L. F., Tabin C. J., Shih C., Weinberg R. A. Human EJ bladder carcinoma oncogene is homologue of Harvey sarcoma virus ras gene. Nature. 1982 Jun 10;297(5866):474–478. doi: 10.1038/297474a0. [DOI] [PubMed] [Google Scholar]
  20. Pravtcheva D. D., Ruddle F. H., Ellis R. W., Scolnick E. M. Assignment of murine cellular Harvey ras gene to chromosome 7. Somatic Cell Genet. 1983 Nov;9(6):681–686. doi: 10.1007/BF01539473. [DOI] [PubMed] [Google Scholar]
  21. Pulciani S., Santos E., Lauver A. V., Long L. K., Aaronson S. A., Barbacid M. Oncogenes in solid human tumours. Nature. 1982 Dec 9;300(5892):539–542. doi: 10.1038/300539a0. [DOI] [PubMed] [Google Scholar]
  22. Rabin M., Watson M., Barker P. E., Ryan J., Breg W. R., Ruddle F. H. NRAS transforming gene maps to region p11----p13 on chromosome 1 by in situ hybridization. Cytogenet Cell Genet. 1984;38(1):70–72. doi: 10.1159/000132032. [DOI] [PubMed] [Google Scholar]
  23. Reddy E. P., Reynolds R. K., Santos E., Barbacid M. A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene. Nature. 1982 Nov 11;300(5888):149–152. doi: 10.1038/300149a0. [DOI] [PubMed] [Google Scholar]
  24. Ruley H. E. Adenovirus early region 1A enables viral and cellular transforming genes to transform primary cells in culture. Nature. 1983 Aug 18;304(5927):602–606. doi: 10.1038/304602a0. [DOI] [PubMed] [Google Scholar]
  25. Ryan J., Barker P. E., Shimizu K., Wigler M., Ruddle F. H. Chromosomal assignment of a family of human oncogenes. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4460–4463. doi: 10.1073/pnas.80.14.4460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Santos E., Martin-Zanca D., Reddy E. P., Pierotti M. A., Della Porta G., Barbacid M. Malignant activation of a K-ras oncogene in lung carcinoma but not in normal tissue of the same patient. Science. 1984 Feb 17;223(4637):661–664. doi: 10.1126/science.6695174. [DOI] [PubMed] [Google Scholar]
  27. Santos E., Tronick S. R., Aaronson S. A., Pulciani S., Barbacid M. T24 human bladder carcinoma oncogene is an activated form of the normal human homologue of BALB- and Harvey-MSV transforming genes. Nature. 1982 Jul 22;298(5872):343–347. doi: 10.1038/298343a0. [DOI] [PubMed] [Google Scholar]
  28. Schwab M., Alitalo K., Varmus H. E., Bishop J. M., George D. A cellular oncogene (c-Ki-ras) is amplified, overexpressed, and located within karyotypic abnormalities in mouse adrenocortical tumour cells. Nature. 1983 Jun 9;303(5917):497–501. doi: 10.1038/303497a0. [DOI] [PubMed] [Google Scholar]
  29. Shimizu K., Goldfarb M., Perucho M., Wigler M. Isolation and preliminary characterization of the transforming gene of a human neuroblastoma cell line. Proc Natl Acad Sci U S A. 1983 Jan;80(2):383–387. doi: 10.1073/pnas.80.2.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Shimizu K., Goldfarb M., Suard Y., Perucho M., Li Y., Kamata T., Feramisco J., Stavnezer E., Fogh J., Wigler M. H. Three human transforming genes are related to the viral ras oncogenes. Proc Natl Acad Sci U S A. 1983 Apr;80(8):2112–2116. doi: 10.1073/pnas.80.8.2112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  32. Sukumar S., Pulciani S., Doniger J., DiPaolo J. A., Evans C. H., Zbar B., Barbacid M. A transforming ras gene in tumorigenic guinea pig cell lines initiated by diverse chemical carcinogens. Science. 1984 Mar 16;223(4641):1197–1199. doi: 10.1126/science.6322298. [DOI] [PubMed] [Google Scholar]
  33. Tabin C. J., Bradley S. M., Bargmann C. I., Weinberg R. A., Papageorge A. G., Scolnick E. M., Dhar R., Lowy D. R., Chang E. H. Mechanism of activation of a human oncogene. Nature. 1982 Nov 11;300(5888):143–149. doi: 10.1038/300143a0. [DOI] [PubMed] [Google Scholar]
  34. Taparowsky E., Shimizu K., Goldfarb M., Wigler M. Structure and activation of the human N-ras gene. Cell. 1983 Sep;34(2):581–586. doi: 10.1016/0092-8674(83)90390-2. [DOI] [PubMed] [Google Scholar]
  35. Yuasa Y., Srivastava S. K., Dunn C. Y., Rhim J. S., Reddy E. P., Aaronson S. A. Acquisition of transforming properties by alternative point mutations within c-bas/has human proto-oncogene. Nature. 1983 Jun 30;303(5920):775–779. doi: 10.1038/303775a0. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES