Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1984 Aug 10;12(15):6073–6090. doi: 10.1093/nar/12.15.6073

Subfamilies of the mouse major urinary protein (MUP) multi-gene family: sequence analysis of cDNA clones and differential regulation in the liver.

N J Kuhn, M Woodworth-Gutai, K W Gross, W A Held
PMCID: PMC320058  PMID: 6548015

Abstract

The mouse major urinary proteins (MUPs) are the products of a multi-gene family of 30-35 genes whose members exhibit diverse tissue specific, developmental, and hormonal controls. Three cDNA clones corresponding to liver MUP mRNAs have been sequenced. Two of the clones (p499, C57BL/6 and p1057, BALB/c) share strong homology whereas a third clone (p199, C57BL/6) has diverged considerably from the others at the nucleic acid (85% homology) and protein (68% homology) levels. The 5' regions of p499 and p199 which show the most sequence divergence were subcloned and shown to hybridize to different liver MUP mRNAs. The p499-5' sequence was expressed in all MUP expressing tissues (liver, lachrymal, submaxillary and mammary) whereas the p199-5' sequence was expressed primarily in the liver and lachrymal. Analysis of liver RNA from mice in different endocrine states indicates that the p499-5' sequence is strongly regulated by thyroxine administration whereas the p199-5' sequence is not. Both sequences appear to be regulated by growth hormone and testosterone. Southern blot analysis of mouse genomic DNA indicates that there are multiple genes homologous to each sequence.

Full text

PDF
6073

Images in this article

6080Image
on p.6080
6081Image
on p.6081
6082Image
on p.6082
6084Image
on p.6084

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barth R. K., Gross K. W., Gremke L. C., Hastie N. D. Developmentally regulated mRNAs in mouse liver. Proc Natl Acad Sci U S A. 1982 Jan;79(2):500–504. doi: 10.1073/pnas.79.2.500. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baumann H., Held W. A. Biosynthesis and hormone-regulated expression of secretory glycoproteins in rat liver and hepatoma cells. Effect of glucocorticoids and inflammation. J Biol Chem. 1981 Oct 10;256(19):10145–10155. [PubMed] [Google Scholar]
  3. Bennett K. L., Lalley P. A., Barth R. K., Hastie N. D. Mapping the structural genes coding for the major urinary proteins in the mouse: combined use of recombinant inbred strains and somatic cell hybrids. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1220–1224. doi: 10.1073/pnas.79.4.1220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berger F. G., Gross K. W., Watson G. Isolation and characterization of a DNA sequence complementary to an androgen-inducible messenger RNA from mouse kidney. J Biol Chem. 1981 Jul 10;256(13):7006–7013. [PubMed] [Google Scholar]
  5. Bishop J. O., Clark A. J., Clissold P. M., Hainey S., Francke U. Two main groups of mouse major urinary protein genes, both largely located on chromosome 4. EMBO J. 1982;1(5):615–620. doi: 10.1002/j.1460-2075.1982.tb01217.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  7. Clark A. J., Clissold P. M., Bishop J. O. Variation between mouse major urinary protein genes isolated from a single inbred line. Gene. 1982 Jun;18(3):221–230. doi: 10.1016/0378-1119(82)90159-7. [DOI] [PubMed] [Google Scholar]
  8. Dolan K. P., Unterman R., McLaughlin M., Nakhasi H. L., Lynch K. R., Feigelson P. The structure and expression of very closely related members of the alpha 2u globulin gene family. J Biol Chem. 1982 Nov 25;257(22):13527–13534. [PubMed] [Google Scholar]
  9. Hastie N. D., Held W. A., Toole J. J. Multiple genes coding for the androgen-regulated major urinary proteins of the mouse. Cell. 1979 Jun;17(2):449–457. doi: 10.1016/0092-8674(79)90171-5. [DOI] [PubMed] [Google Scholar]
  10. Held W. A., West K., Gallagher J. F. Importance of initiation factor preparations in the translation of reovirus and globin mRNAs lacking a 5'-terminal 7-methylguanosine. J Biol Chem. 1977 Dec 10;252(23):8489–8497. [PubMed] [Google Scholar]
  11. Kedes L. H. Histone genes and histone messengers. Annu Rev Biochem. 1979;48:837–870. doi: 10.1146/annurev.bi.48.070179.004201. [DOI] [PubMed] [Google Scholar]
  12. Knopf J. L., Gallagher J. F., Held W. A. Differential, multihormonal regulation of the mouse major urinary protein gene family in the liver. Mol Cell Biol. 1983 Dec;3(12):2232–2240. doi: 10.1128/mcb.3.12.2232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Krauter K., Leinwand L., D'Eustachio P., Ruddle F., Darnell J. E., Jr Structural genes of the mouse major urinary protein are on chromosome 4. J Cell Biol. 1982 Aug;94(2):414–417. doi: 10.1083/jcb.94.2.414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kurtz D. T., Feigelson P. Multihormonal induction of hepatic alpha2u-globulin mRNA as measured by hybridization to complementary DNA. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4791–4795. doi: 10.1073/pnas.74.11.4791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kurtz D. T. Rat alpha 2u globulin is encoded by a multigene family. J Mol Appl Genet. 1981;1(1):29–38. [PubMed] [Google Scholar]
  16. Laperche Y., Lynch K. R., Dolan K. P., Feigelson P. Tissue-specific control of alpha 2u globulin gene expression: constitutive synthesis in the submaxillary gland. Cell. 1983 Feb;32(2):453–460. doi: 10.1016/0092-8674(83)90465-8. [DOI] [PubMed] [Google Scholar]
  17. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  19. Roy A. K., Dowbenko D. J. Role of growth hormone in the multihormonal regulation of messenger RNA for alpha2u globulin in the liver of hypophysectomized rats. Biochemistry. 1977 Aug 23;16(17):3918–3922. doi: 10.1021/bi00636a030. [DOI] [PubMed] [Google Scholar]
  20. Sanger F., Coulson A. R. The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978 Mar 1;87(1):107–110. doi: 10.1016/0014-5793(78)80145-8. [DOI] [PubMed] [Google Scholar]
  21. Shaw P. H., Held W. A., Hastie N. D. The gene family for major urinary proteins: expression in several secretory tissues of the mouse. Cell. 1983 Mar;32(3):755–761. doi: 10.1016/0092-8674(83)90061-2. [DOI] [PubMed] [Google Scholar]
  22. Spritz R. A., DeRiel J. K., Forget B. G., Weissman S. M. Complete nucleotide sequence of the human delta-globin gene. Cell. 1980 Oct;21(3):639–646. doi: 10.1016/0092-8674(80)90427-4. [DOI] [PubMed] [Google Scholar]
  23. Szoka P. R., Gallagher J. F., Held W. A. In vitro synthesis and characterization of precursors to the mouse major urinary proteins. J Biol Chem. 1980 Feb 25;255(4):1367–1373. [PubMed] [Google Scholar]
  24. Unterman R. D., Lynch K. R., Nakhasi H. L., Dolan K. P., Hamilton J. W., Cohn D. V., Feigelson P. Cloning and sequence of several alpha 2u-globulin cDNAs. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3478–3482. doi: 10.1073/pnas.78.6.3478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Woodworth-Gutai M. Recombination in SV40-infected cells: nucleotide sequences at viral-viral recombinant joints in naturally arising variants. Virology. 1981 Mar;109(2):344–352. doi: 10.1016/0042-6822(81)90505-5. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES