Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1984 Sep 11;12(17):6741–6749. doi: 10.1093/nar/12.17.6741

Locations and sequences of tobacco chloroplast genes for tRNAPro(UGG), tRNATrp, tRNAfMet and tRNAGly(GCC): the tRNAGly contains only two base-pairs in the D stem.

M Ohme, T Kamogashira, K Shinozoki, M Sugiura
PMCID: PMC320113  PMID: 6091037

Abstract

The nucleotide sequences of tobacco chloroplast genes for tRNAPro(UGG), tRNATrp, tRNAfMet and tRNAGly(GCC) have been determined. None of these genes contains an intron. One unusual feature is that the tRNAGly contains only two base-pairs (A-U, G-U) in the D stem. These four tRNA genes were located in the known physical map of tobacco chloroplast DNA. Hybridization analysis to chloroplast tRNA revealed that all four tRNA genes are transcribed in vivo.

Full text

PDF
6741

Images in this article

6748Image
on p.6748

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Calagan J. L., Pirtle R. M., Pirtle I. L., Kashdan M. A., Vreman H. J., Dudock B. S. Homology between chloroplast and prokaryotic initiator tRNA. Nucleotide sequence of spinach chloroplast methionine initiator tRNA. J Biol Chem. 1980 Oct 25;255(20):9981–9984. [PubMed] [Google Scholar]
  2. Canaday J., Guillemaut P., Gloeckler R., Weil J. H. The nucleotide sequence of spinach chloroplast tryptophan transfer RNA. Nucleic Acids Res. 1981 Jan 10;9(1):47–53. doi: 10.1093/nar/9.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Deno H., Kato A., Shinozaki K., Sugiura M. Nucleotide sequences of tobacco chloroplast genes for elongator tRNAMet and tRNAVal (UAC): the tRNAVal (UAC) gene contains a long intron. Nucleic Acids Res. 1982 Dec 11;10(23):7511–7520. doi: 10.1093/nar/10.23.7511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Deno H., Sugiura M. Chloroplast tRNA gene contains a long intron in the D stem: Nucleotide sequences of tobacco chloroplast genes for tRNA (UCC) and tRNA (UCU). Proc Natl Acad Sci U S A. 1984 Jan;81(2):405–408. doi: 10.1073/pnas.81.2.405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Driesel A. J., Crouse E. J., Gordon K., Bohnert H. J., Herrmann R. G., Steinmetz A., Mubumbila M., Keller M., Burkard G., Weil J. H. Fractionation and identification of spinach chloroplast transfer RNAs and mapping of their genes on the restriction map of chloroplast DNA. Gene. 1979 Aug;6(4):285–306. doi: 10.1016/0378-1119(79)90070-2. [DOI] [PubMed] [Google Scholar]
  6. Francis M., Kashdan M., Sprouse H., Otis L., Dudock B. Nucleotide sequence of a spinach chloroplast proline tRNA. Nucleic Acids Res. 1982 Apr 24;10(8):2755–2758. doi: 10.1093/nar/10.8.2755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hollingsworth M. J., Hallick R. B. Euglena gracilis chloroplast transfer RNA transcription units. Nucleotide sequence analysis of a tRNATyr-tRNAHis-tRNAMet-tRNATrp-tRNAGlu-tRNAGly gene cluster. J Biol Chem. 1982 Nov 10;257(21):12795–12799. [PubMed] [Google Scholar]
  8. Karabin G. D., Hallick R. B. Euglena gracilis chloroplast transfer RNA transcription units. Nucleotide sequence analysis of a tRNAThr-tRNAGly-tRNAMet-tRNASer-tRNAGln gene cluster. J Biol Chem. 1983 May 10;258(9):5512–5518. [PubMed] [Google Scholar]
  9. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Shinozaki K., Deno H., Kato A., Sugiura M. Overlap and cotranscription of the genes for the beta and epsilon subunits of tobacco chloroplast ATPase. Gene. 1983 Oct;24(2-3):147–155. doi: 10.1016/0378-1119(83)90074-4. [DOI] [PubMed] [Google Scholar]
  11. Shinozaki K., Sugiura M. The nucleotide sequence of the tobacco chloroplast gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Gene. 1982 Nov;20(1):91–102. doi: 10.1016/0378-1119(82)90090-7. [DOI] [PubMed] [Google Scholar]
  12. Sprinzl M., Gauss D. H. Compilation of sequences of tRNA genes. Nucleic Acids Res. 1984;12 (Suppl):r59–131. doi: 10.1093/nar/12.suppl.r59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sprinzl M., Gauss D. H. Compilation of tRNA sequences. Nucleic Acids Res. 1984;12 (Suppl):r1–57. [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES