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. 1984 Sep 11;12(17):6797–6812. doi: 10.1093/nar/12.17.6797

Cloning with tandem gene systems for high level gene expression.

N Lee, J Cozzitorto, N Wainwright, D Testa
PMCID: PMC320117  PMID: 6091039

Abstract

A method has been devised for increasing the copy number of a gene (or genes) cloned into a plasmid while minimizing the size of the plasmid. If n copies of a transcriptional unit are cloned, including the promoter, coding region and terminator, the size of the plasmid will increase by n times the total size of the unit. However, if we borrow the concept of polycistronic operon and sandwich n structural genes, each with its own ribosome binding-site, between a promoter and a transcription terminator, there will be a space saving equivalent to n-1 promoters and n-1 transcription terminators. We have constructed plasmids in which an E. coli lipoprotein promoter is followed by 1 to 4 human leukocyte interferon genes and a transcription terminator. The applications of this method in genetic engineering are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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