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. 1984 Sep 11;12(17):6887–6900. doi: 10.1093/nar/12.17.6887

Analysis of immunoglobulin heavy chain V-region genes belonging to the V NP-gene family.

T Blankenstein, G Zoebelein, U Krawinkel
PMCID: PMC320124  PMID: 6091043

Abstract

A method was devised to clone immunoglobulin VH-region genes located on selected restriction fragments from genomic DNA directly into M13 vectors for subsequent nucleotide sequence analysis. Ten recombinant M13 clones representing four so far unknown VH-region genes of the VNP-gene family have been analysed. Sequence comparison shows that these genes are closely related to other VH-genes of the VNP gene family. One of the VH-genes exhibits a so far unobserved unusual length of 100 2/3 codons and appears to be functional. Analysis of the variation of the isolated VH-genes suggests that framework and complementarity determining regions are exposed to separate types of selective pressures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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