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. 1984 Sep 25;12(18):6925–6934. doi: 10.1093/nar/12.18.6925

Molecular cloning and the complete nucleotide sequence of the creatine kinase-M cDNA from chicken.

R W Kwiatkowski, C W Schweinfest, R P Dottin
PMCID: PMC320133  PMID: 6091045

Abstract

The nucleotide sequence of creatine kinase-M (CK-M) cDNA clones has been determined. It includes the entire coding region of 381 amino acids in addition to 5' and 3' untranslated regions. A comparison with a partial sequence from rat CK-M reveals 84% nucleotide sequence homology in the coding region but divergence in the 3' untranslated region. The amino acid sequence is 94% conserved between chicken and rat. Hybridization to RNA immobilized on filters indicates homology between the CK-M 3' untranslated region and additional muscle specific RNA species. The coding region hybridizes only to CK-M RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Biggin M. D., Gibson T. J., Hong G. F. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963–3965. doi: 10.1073/pnas.80.13.3963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Caravatti M., Perriard J. C., Eppenberger H. M. Developmental regulation of creatine kinase isoenzymes in myogenic cell cultures from chicken. Biosynthesis of creatine kinase subunits M and B. J Biol Chem. 1979 Feb 25;254(4):1388–1394. [PubMed] [Google Scholar]
  3. Chung S., Landfear S. M., Blumberg D. D., Cohen N. S., Lodish H. F. Synthesis and stability of developmentally regulated dictyostelium mRNAs are affected by cell--cell contact and cAMP. Cell. 1981 Jun;24(3):785–797. doi: 10.1016/0092-8674(81)90104-5. [DOI] [PubMed] [Google Scholar]
  4. Costantini F. D., Britten R. J., Davidson E. H. Message sequences and short repetitive sequences are interspersed in sea urchin egg poly(A)+ RNAs. Nature. 1980 Sep 11;287(5778):111–117. doi: 10.1038/287111a0. [DOI] [PubMed] [Google Scholar]
  5. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  6. Dym H., Yaffe D. Expression of creatine kinase isoenzymes in myogenic cell lines. Dev Biol. 1979 Feb;68(2):592–599. doi: 10.1016/0012-1606(79)90229-x. [DOI] [PubMed] [Google Scholar]
  7. Eppenberger H. M., Dawson D. M., Kaplan N. O. The comparative enzymology of creatine kinases. I. Isolation and characterization from chicken and rabbit tissues. J Biol Chem. 1967 Jan 25;242(2):204–209. [PubMed] [Google Scholar]
  8. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hastings K. E., Emerson C. P., Jr Codon usage in muscle genes and liver genes. J Mol Evol. 1983;19(3-4):214–218. doi: 10.1007/BF02099968. [DOI] [PubMed] [Google Scholar]
  10. Jukes T. H. Silent nucleotide substitutions and the molecular evolutionary clock. Science. 1980 Nov 28;210(4473):973–978. doi: 10.1126/science.7434017. [DOI] [PubMed] [Google Scholar]
  11. Kozak M. Point mutations close to the AUG initiator codon affect the efficiency of translation of rat preproinsulin in vivo. Nature. 1984 Mar 15;308(5956):241–246. doi: 10.1038/308241a0. [DOI] [PubMed] [Google Scholar]
  12. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  13. Perriard J. C., Caravatti M., Perriard E. R., Eppenberger H. M. Quantitation of creatine kinase isoenzyme transition in differentiating chicken embryonic breast muscle and myogenic cell cultures by immunoadsorption. Arch Biochem Biophys. 1978 Nov;191(1):90–100. doi: 10.1016/0003-9861(78)90070-x. [DOI] [PubMed] [Google Scholar]
  14. Perryman M. B., Strauss A. W., Buettner T. L., Roberts R. Molecular heterogeneity of creatine kinase isoenzymes. Biochim Biophys Acta. 1983 Sep 28;747(3):284–290. doi: 10.1016/0167-4838(83)90107-3. [DOI] [PubMed] [Google Scholar]
  15. Proudfoot N. J., Brownlee G. G. 3' non-coding region sequences in eukaryotic messenger RNA. Nature. 1976 Sep 16;263(5574):211–214. doi: 10.1038/263211a0. [DOI] [PubMed] [Google Scholar]
  16. Rave N., Crkvenjakov R., Boedtker H. Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels. Nucleic Acids Res. 1979 Aug 10;6(11):3559–3567. doi: 10.1093/nar/6.11.3559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  18. Rosenberg U. B., Kunz G., Frischauf A., Lehrach H., Mähr R., Eppenberger H. M., Perriard J. C. Molecular cloning and expression during myogenesis of sequences coding for M-creatine kinase. Proc Natl Acad Sci U S A. 1982 Nov;79(21):6589–6592. doi: 10.1073/pnas.79.21.6589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Roy B. P. The amino acid sequence of the peptide containing the thiol group of creatine kinase from normal and dystrophic chicken breast muscle. Comparison of some of the immunological properties of the antibodies developed in rabbits against these enzymes. Biochem J. 1974 Oct;143(1):171–179. doi: 10.1042/bj1430171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schweinfest C. W., Kwiatkowski R. W., Dottin R. P. Molecular cloning of a DNA sequence complementary to creatine kinase M mRNA from chickens. Proc Natl Acad Sci U S A. 1982 Aug;79(16):4997–5000. doi: 10.1073/pnas.79.16.4997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sutcliffe J. G., Milner R. J., Gottesfeld J. M., Lerner R. A. Identifier sequences are transcribed specifically in brain. Nature. 1984 Mar 15;308(5956):237–241. doi: 10.1038/308237a0. [DOI] [PubMed] [Google Scholar]
  23. Tchurikov N. A., Naumova A. K., Zelentsova E. S., Georgiev G. P. A cloned unique gene of Drosophila melanogaster contains a repetitive 3' exon whose sequence is present at the 3' ends of many different mRNAs. Cell. 1982 Feb;28(2):365–373. doi: 10.1016/0092-8674(82)90354-3. [DOI] [PubMed] [Google Scholar]
  24. Wilson A. C., Carlson S. S., White T. J. Biochemical evolution. Annu Rev Biochem. 1977;46:573–639. doi: 10.1146/annurev.bi.46.070177.003041. [DOI] [PubMed] [Google Scholar]

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