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. 1981 Aug;78(8):4758–4762. doi: 10.1073/pnas.78.8.4758

Synthesis of capped and uncapped methylated oligonucleotides by the virion transcriptase of spring viremia of carp virus, a rhabdovirus.

K C Gupta, P Roy
PMCID: PMC320242  PMID: 6946423

Abstract

Capped and uncapped methylated oligonucleotides, five and six nucleotides in length, were synthesized in vitro in spring viremia of carp virus transcription reaction mixtures containing ATP + CTP, ATP + CTP + GTP, or ATP + CTP + UTP, but not in any other combinations of two or three ribonucleoside triphosphates. The oligonucleotides that have been characterized are consistent with the structures: m7GpppAmpNpCpNpN, GpppAmpNpCpNpN, pppAmpNpCpNpN, and ppAmpNpCpNpN--i.e., similar to those of the termini of transcripts made in complete reaction mixtures. Because both capped and uncapped methylated oligonucleotides were synthesized, it can be concluded that methylation of the penultimate nucleotide can precede capping and methylation of the capping nucleotide. Our results also indicate that capping and methylation are processes that can take place prior to mRNA chain completion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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