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. 1981 Aug;78(8):5113–5117. doi: 10.1073/pnas.78.8.5113

B-lymphocyte responses to trinitrophenyl-conjugated Ficoll: requirement for T lymphocytes and Ia-bearing adherent cells.

N L Letvin, B Benacerraf, R N Germain
PMCID: PMC320343  PMID: 6975477

Abstract

These studies were done to characterize the cellular requirements for B-lymphocyte responses to the haptenated polysaccharide trinitrophenyl-conjugated Ficoll. By using an in vitro microculture system, it was demonstrated that hapten-specific anti-trinitrophenyl-conjugated Ficoll plaque-forming cell responses by B lymphocytes require Ia-bearing adherent accessory cells and Thy 1+ Lyt 1+2- nylon wool-nonadherent (T) lymphocytes. Such T cells could be primed in vivo with nonderivatized Ficoll to show carrier-specific helper cell function for derivatized Ficoll responses in vitro. The implications of these findings for our understanding of b-lymphocyte triggering by so-called T-independent antigens are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker P. J., Stashak P. W., Amsbaugh D. F., Prescott B., Barth R. F. Evidence for the existence of two functionally distinct types of cells which regulate the antibody response to type 3 pneumococcal polysaccharide. J Immunol. 1970 Dec;105(6):1581–1583. [PubMed] [Google Scholar]
  2. Baker P. J., Stashak P. W., Amsbaugh D. F., Prescott B. Regulation of the antibody response to type 3 pneumococcal polysaccharide. IV. Role of suppressor T cells in the development of low-dose paralysis. J Immunol. 1974 Jun;112(6):2020–2027. [PubMed] [Google Scholar]
  3. Boswell H. S., Ahmed A., Scher I., Singer A. Role of accessory cells in B cell activation. II. The interaction of B cells with accessory cells results in the exclusive activation of an Lyb5+ B cell subpopulation. J Immunol. 1980 Sep;125(3):1340–1348. [PubMed] [Google Scholar]
  4. Boswell H. S., Sharrow S. O., Singer A. Role of accessory cells in B cell activation. I. Macrophage presentation of TNP-Ficoll: evidence for macrophage-B cell interaction. J Immunol. 1980 Feb;124(2):989–996. [PubMed] [Google Scholar]
  5. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  6. Kirkland T. N., Sieckmann D. G., Longo D. L., Mosier D. E. Cellular requirements for antigen presentation in the induction of a thymus-independent antibody response in vitro. J Immunol. 1980 Apr;124(4):1721–1726. [PubMed] [Google Scholar]
  7. Letvin N. L., Greene M. I., Benacerraf B., Germain R. N. Immunologic effects of whole-body ultraviolet irradiation: selective defect in splenic adherent cell function in vitro. Proc Natl Acad Sci U S A. 1980 May;77(5):2881–2885. doi: 10.1073/pnas.77.5.2881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Marshak-Rothstein A., Fink P., Gridley T., Raulet D. H., Bevan M. J., Gefter M. L. Properties and applications of monoclonal antibodies directed against determinants of the Thy-1 locus. J Immunol. 1979 Jun;122(6):2491–2497. [PubMed] [Google Scholar]
  9. Mond J. J., Mongini P. K., Sieckmann D., Paul W. E. Role of T lymphocytes in the response to TNP-AECM-Ficoll. J Immunol. 1980 Sep;125(3):1066–1070. [PubMed] [Google Scholar]
  10. Mond J. J., Stein K. E., Subbarao B., Paul W. E. Analysis of B cell activation requirements with TNP-conjugated polyacrylamide beads. J Immunol. 1979 Jul;123(1):239–245. [PubMed] [Google Scholar]
  11. Mosier D. E., Johnson B. M., Paul W. E., McMaster P. R. Cellular requirements for the primary in vitro antibody response to DNP-ficoll. J Exp Med. 1974 May 1;139(5):1354–1360. doi: 10.1084/jem.139.5.1354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mosier D. E., Mond J. J., Goldings E. A. The ontogeny of thymic independent antibody responses in vitro in normal mice and mice with an X-linked B cell defect. J Immunol. 1977 Dec;119(6):1874–1878. [PubMed] [Google Scholar]
  13. Raff M. C. Theta-bearing lymphocytes in nude mice. Nature. 1973 Dec 7;246(5432):350–351. doi: 10.1038/246350a0. [DOI] [PubMed] [Google Scholar]
  14. Schott C. F., Merchant B. Carrier-specific immune memory to a thymus-independent antigen in congenitally athymic mice. J Immunol. 1979 May;122(5):1710–1718. [PubMed] [Google Scholar]
  15. Weinberger J. Z., Germain R. N., Benacerraf B., Dorf M. E. Hapten-specific T cell responses to 4-hydroxy-3-nitrophenyl acetyl. V. Role of idiotypes in the suppressor pathway. J Exp Med. 1980 Jul 1;152(1):161–169. doi: 10.1084/jem.152.1.161. [DOI] [PMC free article] [PubMed] [Google Scholar]

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