Abstract
A series of monoclonal antibodies has been raised against the human choriocarcinoma cell-line, BeWo. Four antigens, Trop-1, -2, -3, and -4, are defined on normal and malignant trophoblast cells. Trop-1 and Trop-2 appear to be specifically expressed on syncytio- and cytotrophoblasts, whereas Trop-3 and Trop-4 are also detected on various tumor cell lines, normal lymphocytes, and monocytes. Anti-Trop-1 and anti-Trop-2 antibodies might prove useful for detection and isolation of fetal trophoblast cells circulating in pregnant women's blood and for diagnosis and therapy in patients having choriocarcinomas and other germ-cell neoplasms.
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- Beer A. E., Billingham R. E., Yang S. L. Further evidence concerning the autoantigenic status of the trophoblast. J Exp Med. 1972 May 1;135(5):1177–1184. doi: 10.1084/jem.135.5.1177. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Faulk W. P., Temple A., Lovins R. E., Smith N. Antigens of human trophoblasts: a working hypothesis for their role in normal and abnormal pregnancies. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1947–1951. doi: 10.1073/pnas.75.4.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Herzenberg L. A., Bianchi D. W., Schröder J., Cann H. M., Iverson G. M. Fetal cells in the blood of pregnant women: detection and enrichment by fluorescence-activated cell sorting. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1453–1455. doi: 10.1073/pnas.76.3.1453. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Iverson G. M., Bianchi D. W., Cann H. M., Herzenberg L. A. Detection and isolation of fetal cells from maternal blood using the flourescence-activated cell sorter (FACS). Prenat Diagn. 1981 Jan;1(1):61–73. doi: 10.1002/pd.1970010111. [DOI] [PubMed] [Google Scholar]
- Krolick K. A., Villemez C., Isakson P., Uhr J. W., Vitetta E. S. Selective killing of normal or neoplastic B cells by antibodies coupled to the A chain of ricin. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5419–5423. doi: 10.1073/pnas.77.9.5419. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
- Oi V. T., Herzenberg L. A. Localization of murine Ig-1b and Ig-1a (IgG 2a) allotypic determinants detected with monoclonal antibodies. Mol Immunol. 1979 Dec;16(12):1005–1017. doi: 10.1016/0161-5890(79)90034-8. [DOI] [PubMed] [Google Scholar]
- PARK W. W. Experimental trophoblastic embolism of the lungs. J Pathol Bacteriol. 1958 Apr;75(2):257–265. doi: 10.1002/path.1700750204. [DOI] [PubMed] [Google Scholar]
- Parks D. R., Bryan V. M., Oi V. T., Herzenberg L. A. Antigen-specific identification and cloning of hybridomas with a fluorescence-activated cell sorter. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1962–1966. doi: 10.1073/pnas.76.4.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rouse R. V., van Ewijk W., Jones P. P., Weissman I. L. Expression of MHC antigens by mouse thymic dendritic cells. J Immunol. 1979 Jun;122(6):2508–2515. [PubMed] [Google Scholar]
- Schröder J. Transplacental passage of blood cells. J Med Genet. 1975 Sep;12(3):230–242. [PMC free article] [PubMed] [Google Scholar]
- Youle R. J., Neville D. M., Jr Anti-Thy 1.2 monoclonal antibody linked to ricin is a potent cell-type-specific toxin. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5483–5486. doi: 10.1073/pnas.77.9.5483. [DOI] [PMC free article] [PubMed] [Google Scholar]