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. 1984 Nov 26;12(22):8699–8710. doi: 10.1093/nar/12.22.8699

Molecular cloning and sequencing of the reovirus (serotype 3) S1 gene which encodes the viral cell attachment protein sigma 1.

L Nagata, S A Masri, D C Mah, P W Lee
PMCID: PMC320408  PMID: 6095208

Abstract

The complete sequence of the reovirus (serotype 3) S1 gene was obtained by using cloned cDNA derived from the RNA segment. This gene is 1416 nucleotides in length and contains two open reading frames. The first reading frame has a coding capacity of 455 amino acids, sufficient to account for the known S1 product, protein sigma 1 (42,000 MW). It possesses a signal peptide as well as three possible glycosylation sites. No homology could be detected when this gene sequence and the deduced amino acid sequence were compared to published sequences of the corresponding gene of a human rotavirus. The second reading frame (not in phase with the first) starts at the second ATG recently shown to be a functional initiation site. It has a coding capacity of 120 amino acids. Its outstanding feature is the highly basic amino-terminal region, a characteristic apparently shared by a number of DNA binding proteins. It is speculated that this protein, hitherto undetected, may play a role in mediating viral and/or host nucleic acid transcription.

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Selected References

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  1. Antczak J. B., Chmelo R., Pickup D. J., Joklik W. K. Sequence at both termini of the 10 genes of reovirus serotype 3 (strain Dearing). Virology. 1982 Sep;121(2):307–319. doi: 10.1016/0042-6822(82)90170-2. [DOI] [PubMed] [Google Scholar]
  2. Arias C. F., López S., Bell J. R., Strauss J. H. Primary structure of the neutralization antigen of simian rotavirus SA11 as deduced from cDNA sequence. J Virol. 1984 May;50(2):657–661. doi: 10.1128/jvi.50.2.657-661.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Babiss L. E., Luftig R. B., Weatherbee J. A., Weihing R. R., Ray U. R., Fields B. N. Reovirus serotypes 1 and 3 differ in their in vitro association with microtubules. J Virol. 1979 Jun;30(3):863–874. doi: 10.1128/jvi.30.3.863-874.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bridger J. C., Woode G. N. Neonatal calf diarrhoea: identification of a reovirus-like (rotavirus) agent in faeces by immunofluorescence and immune electron microscopy. Br Vet J. 1975 Sep-Oct;131(5):528–535. [PubMed] [Google Scholar]
  6. Burstin S. J., Spriggs D. R., Fields B. N. Evidence for functional domains on the reovirus type 3 hemagglutinin. Virology. 1982 Feb;117(1):146–155. doi: 10.1016/0042-6822(82)90514-1. [DOI] [PubMed] [Google Scholar]
  7. Cashdollar L. W., Esparza J., Hudson G. R., Chmelo R., Lee P. W., Joklik W. K. Cloning the double-stranded RNA genes of reovirus: sequence of the cloned S2 gene. Proc Natl Acad Sci U S A. 1982 Dec;79(24):7644–7648. doi: 10.1073/pnas.79.24.7644. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cenatiempo Y., Twardowski T., Shoeman R., Ernst H., Brot N., Weissbach H., Shatkin A. J. Two initiation sites detected in the small s1 species of reovirus mRNA by dipeptide synthesis in vitro. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1084–1088. doi: 10.1073/pnas.81.4.1084. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Chou P. Y., Fasman G. D. Empirical predictions of protein conformation. Annu Rev Biochem. 1978;47:251–276. doi: 10.1146/annurev.bi.47.070178.001343. [DOI] [PubMed] [Google Scholar]
  10. Dyall-Smith M. L., Holmes I. H. Sequence homology between human and animal rotavirus serotype-specific glycoproteins. Nucleic Acids Res. 1984 May 11;12(9):3973–3982. doi: 10.1093/nar/12.9.3973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Elleman T. C., Hoyne P. A., Dyall-Smith M. L., Holmes I. H., Azad A. A. Nucleotide sequence of the gene encoding the serotype-specific glycoprotein of UK bovine rotavirus. Nucleic Acids Res. 1983 Jul 25;11(14):4689–4701. doi: 10.1093/nar/11.14.4689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Fields B. N., Greene M. I. Genetic and molecular mechanisms of viral pathogenesis: implications for prevention and treatment. Nature. 1982 Nov 4;300(5887):19–23. doi: 10.1038/300019a0. [DOI] [PubMed] [Google Scholar]
  13. Finberg R., Weiner H. L., Fields B. N., Benacerraf B., Burakoff S. J. Generation of cytolytic T lymphocytes after reovirus infection: role of S1 gene. Proc Natl Acad Sci U S A. 1979 Jan;76(1):442–446. doi: 10.1073/pnas.76.1.442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Flewett T. H., Woode G. N. The rotaviruses. Arch Virol. 1978;57(1):1–23. doi: 10.1007/BF01315633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Fontana A., Weiner H. L. Interaction of reovirus with cell surface receptors. II. Generation of suppressor T cells by the hemagglutinin of reovirus type 3. J Immunol. 1980 Dec;125(6):2660–2664. [PubMed] [Google Scholar]
  16. Franklin N. C., Bennett G. N. The N protein of bacteriophage lambda, defined by its DNA sequence, is highly basic. Gene. 1979 Dec;8(1):107–119. doi: 10.1016/0378-1119(79)90011-8. [DOI] [PubMed] [Google Scholar]
  17. Gaillard R. K., Joklik W. K. The antigenic determinants of most of the proteins coded by the three serotypes of reovirus are highly conserved during evolution. Virology. 1980 Dec;107(2):533–536. doi: 10.1016/0042-6822(80)90321-9. [DOI] [PubMed] [Google Scholar]
  18. Gaillard R. K., Jr, Joklik W. K. Quantitation of the relatedness of reovirus serotypes 1, 2, and 3 at the gene level. Virology. 1982 Nov;123(1):152–164. doi: 10.1016/0042-6822(82)90302-6. [DOI] [PubMed] [Google Scholar]
  19. Grosschedl R., Schwarz E. Nucleotide sequence of the cro-cII-oop region of bacteriophage 434 DNA. Nucleic Acids Res. 1979 Mar;6(3):867–881. doi: 10.1093/nar/6.3.867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Hayes E. C., Lee P. W., Miller S. E., Joklik W. K. The interaction of a series of hybridoma IgGs with reovirus particles. Demonstration that the core protein lambda 2 is exposed on the particle surface. Virology. 1981 Jan 15;108(1):147–155. doi: 10.1016/0042-6822(81)90534-1. [DOI] [PubMed] [Google Scholar]
  21. Hopp T. P., Woods K. R. Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3824–3828. doi: 10.1073/pnas.78.6.3824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Huddleston J. A., Brownlee G. G. The sequence of the nucleoprotein gene of human influenza A virus, strain A/NT/60/68. Nucleic Acids Res. 1982 Feb 11;10(3):1029–1038. doi: 10.1093/nar/10.3.1029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Kozak M. Analysis of ribosome binding sites from the s1 message of reovirus. Initiation at the first and second AUG codons. J Mol Biol. 1982 Apr 25;156(4):807–820. doi: 10.1016/0022-2836(82)90143-7. [DOI] [PubMed] [Google Scholar]
  24. Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. doi: 10.1016/0022-2836(82)90515-0. [DOI] [PubMed] [Google Scholar]
  25. Lee P. W., Hayes E. C., Joklik W. K. Characterization of anti-reovirus immunoglobulins secreted by cloned hybridoma cell lines. Virology. 1981 Jan 15;108(1):134–146. doi: 10.1016/0042-6822(81)90533-x. [DOI] [PubMed] [Google Scholar]
  26. Lee P. W., Hayes E. C., Joklik W. K. Protein sigma 1 is the reovirus cell attachment protein. Virology. 1981 Jan 15;108(1):156–163. doi: 10.1016/0042-6822(81)90535-3. [DOI] [PubMed] [Google Scholar]
  27. Luftig R. B., Kilham S. S., Hay A. J., Zweerink H. J., Joklik W. K. An ultrastructural study of virions and cores of reovirus type 3. Virology. 1972 Apr;48(1):170–181. doi: 10.1016/0042-6822(72)90124-9. [DOI] [PubMed] [Google Scholar]
  28. McCrae M. A., Joklik W. K. The nature of the polypeptide encoded by each of the 10 double-stranded RNA segments of reovirus type 3. Virology. 1978 Sep;89(2):578–593. doi: 10.1016/0042-6822(78)90199-x. [DOI] [PubMed] [Google Scholar]
  29. McCrae M. A. Terminal structure of reovirus RNAs. J Gen Virol. 1981 Aug;55(Pt 2):393–403. doi: 10.1099/0022-1317-55-2-393. [DOI] [PubMed] [Google Scholar]
  30. Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Mustoe T. A., Ramig R. F., Sharpe A. H., Fields B. N. A genetic map of reovirus. III. Assignment of the double-stranded RNA-positive mutant groups A, B, and G to genome segments. Virology. 1978 Apr;85(2):545–556. doi: 10.1016/0042-6822(78)90460-9. [DOI] [PubMed] [Google Scholar]
  32. Norrander J., Kempe T., Messing J. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene. 1983 Dec;26(1):101–106. doi: 10.1016/0378-1119(83)90040-9. [DOI] [PubMed] [Google Scholar]
  33. Novotny J. Matrix program to analyze primary structure homology. Nucleic Acids Res. 1982 Jan 11;10(1):127–131. doi: 10.1093/nar/10.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Perlman D., Halvorson H. O. A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides. J Mol Biol. 1983 Jun 25;167(2):391–409. doi: 10.1016/s0022-2836(83)80341-6. [DOI] [PubMed] [Google Scholar]
  35. Pett D. M., Vanaman T. C., Joklik W. K. Studies on the amino and carboxyl terminal amino acid sequences of reovirus capsid polypeptides. Virology. 1973 Mar;52(1):174–186. doi: 10.1016/0042-6822(73)90407-8. [DOI] [PubMed] [Google Scholar]
  36. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  37. Sauer R. T. DNA sequence of the bacteriophage gama cI gene. Nature. 1978 Nov 16;276(5685):301–302. doi: 10.1038/276301a0. [DOI] [PubMed] [Google Scholar]
  38. Sauer R. T., Pan J., Hopper P., Hehir K., Brown J., Poteete A. R. Primary structure of the phage P22 repressor and its gene c2. Biochemistry. 1981 Jun 9;20(12):3591–3598. doi: 10.1021/bi00515a044. [DOI] [PubMed] [Google Scholar]
  39. Sharpe A. H., Fields B. N. Reovirus inhibition of cellular DNA synthesis: role of the S1 gene. J Virol. 1981 Apr;38(1):389–392. doi: 10.1128/jvi.38.1.389-392.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Shaw M. W., Choppin P. W., Lamb R. A. A previously unrecognized influenza B virus glycoprotein from a bicistronic mRNA that also encodes the viral neuraminidase. Proc Natl Acad Sci U S A. 1983 Aug;80(16):4879–4883. doi: 10.1073/pnas.80.16.4879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Smith R. E., Zweerink H. J., Joklik W. K. Polypeptide components of virions, top component and cores of reovirus type 3. Virology. 1969 Dec;39(4):791–810. doi: 10.1016/0042-6822(69)90017-8. [DOI] [PubMed] [Google Scholar]
  42. Spriggs D. R., Kaye K., Fields B. N. Topological analysis of the reovirus type 3 hemagglutinin. Virology. 1983 May;127(1):220–224. doi: 10.1016/0042-6822(83)90385-9. [DOI] [PubMed] [Google Scholar]
  43. Taylor J. M., Illmensee R., Summers J. Efficeint transcription of RNA into DNA by avian sarcoma virus polymerase. Biochim Biophys Acta. 1976 Sep 6;442(3):324–330. doi: 10.1016/0005-2787(76)90307-5. [DOI] [PubMed] [Google Scholar]
  44. Weiner H. L., Drayna D., Averill D. R., Jr, Fields B. N. Molecular basis of reovirus virulence: role of the S1 gene. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5744–5748. doi: 10.1073/pnas.74.12.5744. [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. Weiner H. L., Fields B. N. Neutralization of reovirus: the gene responsible for the neutralization antigen. J Exp Med. 1977 Nov 1;146(5):1305–1310. doi: 10.1084/jem.146.5.1305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Weiner H. L., Greene M. I., Fields B. N. Delayed hypersensitivity in mice infected with reovirus. I. Identification of host and viral gene products responsible for the immune response. J Immunol. 1980 Jul;125(1):278–282. [PubMed] [Google Scholar]

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