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. 1984 Dec 11;12(23):9039–9049. doi: 10.1093/nar/12.23.9039

Structure of the gene coding for the sequence-specific DNA-methyltransferase of the B. subtilis phage SPR.

G Pósfai, F Baldauf, S Erdei, J Pósfai, P Venetianer, A Kiss
PMCID: PMC320436  PMID: 6096817

Abstract

The nucleotide sequence of the gene coding for the 5'-GGCC and 5'-CCGG specific DNA methyltransferase of the Bacillus subtilis phage SPR was determined by the Maxam-Gilbert procedure. Transcriptional and translational signals of the sequence were assigned with the help of S1 mapping and translation in E. coli minicells. The gene codes for a 49 kd polypeptide. The amino acid sequence of the SPR methylase shows regions of homology with the sequence of the 5'-GGCC-specific BspRI modification methylase.

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Selected References

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