Abstract
The phosphite approach to oligodeoxynucleotide synthesis has been applied to the filter disc support. The method is exemplified here by the preparation of eight decadeoxynucleotides.
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Selected References
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- Connolly B. A., Potter B. V., Eckstein F., Pingoud A., Grotjahn L. Synthesis and characterization of an octanucleotide containing the EcoRI recognition sequence with a phosphorothioate group at the cleavage site. Biochemistry. 1984 Jul 17;23(15):3443–3453. doi: 10.1021/bi00310a010. [DOI] [PubMed] [Google Scholar]
- Dörper T., Winnacker E. L. Improvements in the phosphoramidite procedure for the synthesis of oligodeoxyribonucleotides. Nucleic Acids Res. 1983 May 11;11(9):2575–2584. doi: 10.1093/nar/11.9.2575. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Efimov V. A., Reverdatto S. V., Chakhmakhcheva O. G. New effective method for the synthesis of oligonucleotides via phosphotriester intermediates. Nucleic Acids Res. 1982 Nov 11;10(21):6675–6694. doi: 10.1093/nar/10.21.6675. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Frank R., Heikens W., Heisterberg-Moutsis G., Blöcker H. A new general approach for the simultaneous chemical synthesis of large numbers of oligonucleotides: segmental solid supports. Nucleic Acids Res. 1983 Jul 11;11(13):4365–4377. doi: 10.1093/nar/11.13.4365. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gait M. J., Matthes H. W., Singh M., Sproat B. S., Titmas R. C. Rapid synthesis of oligodeoxyribonucleotides. VII. Solid phase synthesis of oligodeoxyribonucleotides by a continuous flow phosphotriester method on a kieselguhr-polyamide support. Nucleic Acids Res. 1982 Oct 25;10(20):6243–6254. doi: 10.1093/nar/10.20.6243. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Itakura K., Rossi J. J., Wallace R. B. Synthesis and use of synthetic oligonucleotides. Annu Rev Biochem. 1984;53:323–356. doi: 10.1146/annurev.bi.53.070184.001543. [DOI] [PubMed] [Google Scholar]
- Matthes H. W., Zenke W. M., Grundström T., Staub A., Wintzerith M., Chambon P. Simultaneous rapid chemical synthesis of over one hundred oligonucleotides on a microscale. EMBO J. 1984 Apr;3(4):801–805. doi: 10.1002/j.1460-2075.1984.tb01888.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Noble S. A., Fisher E. F., Caruthers M. H. Methylphosphonates as probes of protein-nucleic acid interactions. Nucleic Acids Res. 1984 Apr 11;12(7):3387–3404. doi: 10.1093/nar/12.7.3387. [DOI] [PMC free article] [PubMed] [Google Scholar]
