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. 1984 Dec 21;12(24):9271–9285. doi: 10.1093/nar/12.24.9271

DNA structural variations produced by actinomycin and distamycin as revealed by DNAase I footprinting.

K R Fox, M J Waring
PMCID: PMC320460  PMID: 6393053

Abstract

The technique of DNAase I footprinting has been used to investigate preferred binding sites for actinomycin D and distamycin on a 160-base-pair DNA fragment from E. coli containing the tyr T promoter sequence. Only sites containing the dinucleotide step GpC are protected by binding of actinomycin, and all such sites are protected. Distamycin recognizes four major regions rich in A + T residues. Both antibiotics induce enhanced rates of cleavage at certain regions flanking their binding sites. These effects are not restricted to any particular base sequence since they are produced in runs of A and T by actinomycin and in GC-rich sequences by distamycin. The observed increases in susceptibility to nuclease attack are attributed to DNA structural variations induced in the vicinity of the ligand binding site, most probably involving changes in the width of the helical minor groove.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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