Abstract
Four different U2-snRNA genes/related sequences of D. melanogaster were cloned and characterized. The sequences of all four genes suggest that they were generated by a DNA-mediated mechanism. These genes/related sequences were found to be located in two loci, each locus containing two U2 snRNA sequences. Using coding sequences as well as flanking sequences as hybridization probes against polytene chromosomes of D. melanogaster Oregon R we were able to map these loci separately at positions 34BC and 84C. By Northern analysis we observed that the quantities of U2- and U1-snRNA are coordinated and change during the embryonic development of the fly.
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- Alonso A., Jorcano J. L., Beck E., Spiess E. Isolation and characterization of Drosophila melanogaster U2 small nuclear RNA genes. J Mol Biol. 1983 Sep 25;169(3):691–705. doi: 10.1016/s0022-2836(83)80165-x. [DOI] [PubMed] [Google Scholar]
- Beck E., Jorcano J. L., Alonso A. Drosophila melanogaster U1 and U2 small nuclear RNA genes contain common flanking sequences. J Mol Biol. 1984 Mar 15;173(4):539–542. doi: 10.1016/0022-2836(84)90397-8. [DOI] [PubMed] [Google Scholar]
- Blin N., Weber T., Alonso A. Cross-reaction of snRNA and an Alu I-like sequence from rat with DNAs from different eucaryotic species. Nucleic Acids Res. 1983 Mar 11;11(5):1375–1388. doi: 10.1093/nar/11.5.1375. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blin N., Weber T., Alonso A. Cross-reaction of snRNA and an Alu I-like sequence from rat with DNAs from different eucaryotic species. Nucleic Acids Res. 1983 Mar 11;11(5):1375–1388. doi: 10.1093/nar/11.5.1375. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Busch H., Reddy R., Rothblum L., Choi Y. C. SnRNAs, SnRNPs, and RNA processing. Annu Rev Biochem. 1982;51:617–654. doi: 10.1146/annurev.bi.51.070182.003153. [DOI] [PubMed] [Google Scholar]
- Card C. O., Morris G. F., Brown D. T., Marzluff W. F. Sea urchin small nuclear RNA genes are organized in distinct tandemly repeating units. Nucleic Acids Res. 1982 Dec 11;10(23):7677–7688. doi: 10.1093/nar/10.23.7677. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Denison R. A., Weiner A. M. Human U1 RNA pseudogenes may be generated by both DNA- and RNA-mediated mechanisms. Mol Cell Biol. 1982 Jul;2(7):815–828. doi: 10.1128/mcb.2.7.815. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lerner M. R., Boyle J. A., Mount S. M., Wolin S. L., Steitz J. A. Are snRNPs involved in splicing? Nature. 1980 Jan 10;283(5743):220–224. doi: 10.1038/283220a0. [DOI] [PubMed] [Google Scholar]
- Marzluff W. F., Brown D. T., Lobo S., Wang S. S. Isolation and characterization of two linked mouse U1b small nuclear RNA genes. Nucleic Acids Res. 1983 Sep 24;11(18):6255–6270. doi: 10.1093/nar/11.18.6255. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mattaj I. W., Zeller R. Xenopus laevis U2 snRNA genes: tandemly repeated transcription units sharing 5' and 3' flanking homology with other RNA polymerase II transcribed genes. EMBO J. 1983;2(11):1883–1891. doi: 10.1002/j.1460-2075.1983.tb01675.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nojima H., Kornberg R. D. Genes and pseudogenes for mouse U1 and U2 small nuclear RNAs. J Biol Chem. 1983 Jul 10;258(13):8151–8155. [PubMed] [Google Scholar]
- Ohshima Y., Itoh M., Okada N., Miyata T. Novel models for RNA splicing that involve a small nuclear RNA. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4471–4474. doi: 10.1073/pnas.78.7.4471. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Saluz H. P., Schmidt T., Dudler R., Altwegg M., Stumm-Zollinger E., Kubli E., Chen P. S. The genes coding for 4 snRNAs of Drosophila melanogaster: localization and determination of gene numbers. Nucleic Acids Res. 1983 Jan 11;11(1):77–90. doi: 10.1093/nar/11.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Arsdell S. W., Weiner A. M. Human genes for U2 small nuclear RNA are tandemly repeated. Mol Cell Biol. 1984 Mar;4(3):492–499. doi: 10.1128/mcb.4.3.492. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Arsdell S. W., Weiner A. M. Pseudogenes for human U2 small nuclear RNA do not have a fixed site of 3' truncation. Nucleic Acids Res. 1984 Feb 10;12(3):1463–1471. doi: 10.1093/nar/12.3.1463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weiner A. M., Denison R. A. Either gene amplification or gene conversion may maintain the homogeneity of the multigene family encoding human U1 small nuclear RNA. Cold Spring Harb Symp Quant Biol. 1983;47(Pt 2):1141–1149. doi: 10.1101/sqb.1983.047.01.129. [DOI] [PubMed] [Google Scholar]
- Westin G., Zabielski J., Hammarström K., Monstein H. J., Bark C., Pettersson U. Clustered genes for human U2 RNA. Proc Natl Acad Sci U S A. 1984 Jun;81(12):3811–3815. doi: 10.1073/pnas.81.12.3811. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zeller R., Carri M. T., Mattaj I. W., De Robertis E. M. Xenopus laevis U1 snRNA genes: characterisation of transcriptionally active genes reveals major and minor repeated gene families. EMBO J. 1984 May;3(5):1075–1081. doi: 10.1002/j.1460-2075.1984.tb01931.x. [DOI] [PMC free article] [PubMed] [Google Scholar]