Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1985;13(Suppl):r201–r207. doi: 10.1093/nar/13.suppl.r201

The effect of site specific methylation on restriction endonuclease digestion

Michael McClelland 1, Michael Nelson 1
PMCID: PMC320509  PMID: 2987886

Full text

PDF
r201

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Backman K. A cautionary note on the use of certain restriction endonucleases with methylated substrates. Gene. 1980 Oct;11(1-2):169–171. doi: 10.1016/0378-1119(80)90097-9. [DOI] [PubMed] [Google Scholar]
  2. Bingham A. H., Atkinson T., Sciaky D., Roberts R. J. A specific endonuclease from Bacillus caldolyticus. Nucleic Acids Res. 1978 Oct;5(10):3457–3467. doi: 10.1093/nar/5.10.3457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bird A. P., Taggart M. H., Smith B. A. Methylated and unmethylated DNA compartments in the sea urchin genome. Cell. 1979 Aug;17(4):889–901. doi: 10.1016/0092-8674(79)90329-5. [DOI] [PubMed] [Google Scholar]
  4. Boyer H. W., Chow L. T., Dugaiczyk A., Hedgpeth J., Goodman H. M. DNA substrate site for the EcoRII restriction endonuclease and modification methylase. Nat New Biol. 1973 Jul 11;244(132):40–43. doi: 10.1038/newbio244040a0. [DOI] [PubMed] [Google Scholar]
  5. Bron S., Murray K., Trautner T. A. Restriction and modification in B. subtilis. Purification and general properties of a restriction endonuclease from strain R. Mol Gen Genet. 1975 Dec 30;143(1):13–23. doi: 10.1007/BF00269416. [DOI] [PubMed] [Google Scholar]
  6. Busslinger M., deBoer E., Wright S., Grosveld F. G., Flavell R. A. The sequence GGCmCGG is resistant to MspI cleavage. Nucleic Acids Res. 1983 Jun 11;11(11):3559–3569. doi: 10.1093/nar/11.11.3559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dobritsa A. P., Dobritsa S. V. DNA protection with the DNA methylase M . BbvI from Bacillus brevis var. GB against cleavage by the restriction endonucleases PstI and PvuII. Gene. 1980 Jul;10(2):105–112. doi: 10.1016/0378-1119(80)90128-6. [DOI] [PubMed] [Google Scholar]
  8. Dreiseikelmann B., Eichenlaub R., Wackernagel W. The effect of differential methylation by Escherichia coli of plasmid DNA and phage T7 and lambda DNA on the cleavage by restriction endonuclease MboI from Moraxella bovis. Biochim Biophys Acta. 1979 May 24;562(3):418–428. doi: 10.1016/0005-2787(79)90105-9. [DOI] [PubMed] [Google Scholar]
  9. Dugaiczyk A., Hedgpeth J., Boyer H. W., Goodman H. M. Physical identity of the SV40 deoxyribonucleic acid sequence recognized by the Eco RI restriction endonuclease and modification methylase. Biochemistry. 1974 Jan 29;13(3):503–512. doi: 10.1021/bi00700a016. [DOI] [PubMed] [Google Scholar]
  10. Dybvig K., Swinton D., Maniloff J., Hattman S. Cytosine methylation of the sequence GATC in a mycoplasma. J Bacteriol. 1982 Sep;151(3):1420–1424. doi: 10.1128/jb.151.3.1420-1424.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ehrlich M., Wang R. Y. 5-Methylcytosine in eukaryotic DNA. Science. 1981 Jun 19;212(4501):1350–1357. doi: 10.1126/science.6262918. [DOI] [PubMed] [Google Scholar]
  12. Gingeras T. R., Brooks J. E. Cloned restriction/modification system from Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 1983 Jan;80(2):402–406. doi: 10.1073/pnas.80.2.402. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Günthert U., Storm K., Bald R. Restriction and modification in Bacillus subtilis. Localization of the methylated nucleotide in the BsuRI recognition sequence. Eur J Biochem. 1978 Oct 16;90(3):581–583. doi: 10.1111/j.1432-1033.1978.tb12638.x. [DOI] [PubMed] [Google Scholar]
  14. Hattman S., Keister T., Gottehrer A. Sequence specificity of DNA methylases from Bacillus amyloliquefaciens and Bacillus brevis. J Mol Biol. 1978 Oct 5;124(4):701–711. doi: 10.1016/0022-2836(78)90178-x. [DOI] [PubMed] [Google Scholar]
  15. Huang L. H., Farnet C. M., Ehrlich K. C., Ehrlich M. Digestion of highly modified bacteriophage DNA by restriction endonucleases. Nucleic Acids Res. 1982 Mar 11;10(5):1579–1591. doi: 10.1093/nar/10.5.1579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Janulaitis A., Petrusyté M., Butkus V. Three sequence-specific endonucleases from Escherichia coli RFL47. FEBS Lett. 1983 Sep 19;161(2):213–216. doi: 10.1016/0014-5793(83)81010-2. [DOI] [PubMed] [Google Scholar]
  17. Jentsch S., Günthert U., Trautner T. A. DNA methyltransferases affecting the sequence 5'CCGG. Nucleic Acids Res. 1981 Jun 25;9(12):2753–2759. doi: 10.1093/nar/9.12.2753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Jentsch S. Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF. J Bacteriol. 1983 Nov;156(2):800–808. doi: 10.1128/jb.156.2.800-808.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Keshet E., Cedar H. Effect of CpG methylation on Msp I. Nucleic Acids Res. 1983 Jun 11;11(11):3571–3580. doi: 10.1093/nar/11.11.3571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lacks S., Greenberg B. Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation. J Mol Biol. 1977 Jul;114(1):153–168. doi: 10.1016/0022-2836(77)90289-3. [DOI] [PubMed] [Google Scholar]
  21. Lautenberger J. A., Kan N. C., Lackey D., Linn S., Edgell M. H., Hutchison C. A., 3rd Recognition site of Escherichia coli B restriction enzyme on phi XsB1 and simian virus 40 DNAs: an interrupted sequence. Proc Natl Acad Sci U S A. 1978 May;75(5):2271–2275. doi: 10.1073/pnas.75.5.2271. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Lui A. C., McBride B. C., Vovis G. F., Smith M. Site specific endonuclease from Fusobacterium nucleatum. Nucleic Acids Res. 1979 Jan;6(1):1–15. doi: 10.1093/nar/6.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Mann M. B., Smith H. O. Specificity of Hpa II and Hae III DNA methylases. Nucleic Acids Res. 1977 Dec;4(12):4211–4221. doi: 10.1093/nar/4.12.4211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. McClelland M., Kessler L. G., Bittner M. Site-specific cleavage of DNA at 8- and 10-base-pair sequences. Proc Natl Acad Sci U S A. 1984 Feb;81(4):983–987. doi: 10.1073/pnas.81.4.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. McClelland M. The effect of sequence specific DNA methylation on restriction endonuclease cleavage. Nucleic Acids Res. 1981 Nov 25;9(22):5859–5866. doi: 10.1093/nar/9.22.5859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Nelson M., Christ C., Schildkraut I. Alteration of apparent restriction endonuclease recognition specificities by DNA methylases. Nucleic Acids Res. 1984 Jul 11;12(13):5165–5173. doi: 10.1093/nar/12.13.5165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Pirrotta V. Two restriction endonucleases from Bacillus globiggi. Nucleic Acids Res. 1976 Jul;3(7):1747–1760. doi: 10.1093/nar/3.7.1747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Razin A., Urieli S., Pollack Y., Gruenbaum Y., Glaser G. Studies on the biological role of dna methylation; IV. Mode of methylation of DNA in E. coli cells. Nucleic Acids Res. 1980 Apr 25;8(8):1783–1792. doi: 10.1093/nar/8.8.1783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Roberts R. J. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 1984;12 (Suppl):r167–r204. doi: 10.1093/nar/12.suppl.r167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Schlagman S., Hattman S., May M. S., Berger L. In vivo methylation by Escherichia coli K-12 mec+ deoxyribonucleic acid-cytosine methylase protects against in vitro cleavage by the RII restriction endonuclease (R. Eco RII). J Bacteriol. 1976 May;126(2):990–996. doi: 10.1128/jb.126.2.990-996.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Smith H. O., Nathans D. Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes. J Mol Biol. 1973 Dec 15;81(3):419–423. doi: 10.1016/0022-2836(73)90152-6. [DOI] [PubMed] [Google Scholar]
  32. Strobl J. S., Thompson E. B. Methylation of either cytosine in the recognition sequence CGCG inhibits ThaI cleavage of DNA. Nucleic Acids Res. 1984 Nov 12;12(21):8073–8083. doi: 10.1093/nar/12.21.8073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Vovis G. F., Lacks S. Complementary action of restriction enzymes endo R-DpnI and Endo R-DpnII on bacteriophage f1 DNA. J Mol Biol. 1977 Sep 25;115(3):525–538. doi: 10.1016/0022-2836(77)90169-3. [DOI] [PubMed] [Google Scholar]
  34. Waalwijk C., Flavell R. A. MspI, an isoschizomer of hpaII which cleaves both unmethylated and methylated hpaII sites. Nucleic Acids Res. 1978 Sep;5(9):3231–3236. doi: 10.1093/nar/5.9.3231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Walder R. Y., Langtimm C. J., Chatterjee R., Walder J. A. Cloning of the MspI modification enzyme. The site of modification and its effects on cleavage by MspI and HpaII. J Biol Chem. 1983 Jan 25;258(2):1235–1241. [PubMed] [Google Scholar]
  36. Whitehead P. R., Brown N. L. EaeI: a restriction endonuclease from Enterobacter aerogenes. FEBS Lett. 1983 May 2;155(1):97–101. doi: 10.1016/0014-5793(83)80217-8. [DOI] [PubMed] [Google Scholar]
  37. Yoo O. J., Dwyer-Hallquist P., Agarwal K. L. Purification and properties of the Hpa I methylase. Nucleic Acids Res. 1982 Oct 25;10(20):6511–6519. doi: 10.1093/nar/10.20.6511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Youssoufian H., Hammer S. M., Hirsch M. S., Mulder C. Methylation of the viral genome in an in vitro model of herpes simplex virus latency. Proc Natl Acad Sci U S A. 1982 Apr;79(7):2207–2210. doi: 10.1073/pnas.79.7.2207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Youssoufian H., Mulder C. Detection of methylated sequences in eukaryotic DNA with the restriction endonucleases Smai and Xmai. J Mol Biol. 1981 Jul 25;150(1):133–136. doi: 10.1016/0022-2836(81)90328-4. [DOI] [PubMed] [Google Scholar]
  40. van Ormondt H., Lautenberger J. A., Linn S., de Waard A. Methylated oligonucleotides derived from bacteriophage fd RF-DNA modified in vitro by E. coli B modification methylase. FEBS Lett. 1973 Jul 1;33(2):177–180. doi: 10.1016/0014-5793(73)80186-3. [DOI] [PubMed] [Google Scholar]
  41. van der Ploeg L. H., Flavell R. A. DNA methylation in the human gamma delta beta-globin locus in erythroid and nonerythroid tissues. Cell. 1980 Apr;19(4):947–958. doi: 10.1016/0092-8674(80)90086-0. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES