Abstract
Background
In novel treatment approaches, therapeutics should be designed to target cancer stem cells (CSCs). Quantum dots (QDs) are a promising new tool in fighting against cancer. However, little is known about accumulation and cytotoxicity of QDs in CSCs.
Methods
Accumulation and cytotoxicity of CdTe-MPA (mercaptopropionic acid) QDs in CSCs were assessed using flow cytometry and fluorescence-activated cell sorting techniques as well as a colorimetric cell viability assay.
Results
We investigated the expression of two cell surface-associated glycoproteins, CD44 and CD133, in four different cancer cell lines (glioblastoma, melanoma, pancreatic, and prostate adenocarcinoma). Only the melanoma cells were positive to both markers of CD44 and CD133, whereas the other cells were only CD44-positive. The QDs accumulated to a similar extent in all subpopulations of the melanoma cells. The phenotypical response after QD treatment was compared with the response after ionizing radiation treatment. The percentage of the CD44high−CD133high subpopulation decreased from 72% to 55%–58% for both treatments. The stem-like subpopulation CD44highCD133low/− increased from 26%–28% in the untreated melanoma cells to 36%–40% for both treatments.
Conclusion
Treatment of melanoma cells with QDs results in an increase of stem-like cell subpopulations. The changes in phenotype distribution of the melanoma cells after the treatment with QDs are comparable with the changes after ionizing radiation.
Keywords: prominin-1, CD44, glycoproteins, flow cytometry, FACS, nanoparticles
Introduction
Cancer exhibits intratumoral heterogeneity at the genetic and epigenetic levels. The existence of functional heterogeneity between different subpopulations of tumor cells has been described in clonogenic and xenotransplantation studies. Defined subpopulations of cancer cells possess enhanced tumorigenicity when injected into immunodeficient mice. Such isolated cells with self-renewal potential have been termed cancer stem cells (CSCs).1,2
CSCs display extensive differentiation potential and can give rise to new tumors. It has been reported that CSCs are more likely to survive chemotherapy or radiotherapy. Low efficiency of chemotherapy is associated with the ability of CSCs to extrude a drug from the cytosol through transcytosis.3 Other studies have documented that CSCs are also more resistant to radiotherapy than nonstem cells.4 Reduced radiosensitivity is associated with activated defense mechanisms against radiation-induced reactive oxygen species and accelerated repopulation of CSCs. It may also turn out that CSCs are located in hypoxic regions, which shift metabolism to glycolysis and hypoxia- induced radioresistance. The percentage of CSCs within a tumor could also be a predicting factor to radiosensitivity or chemotherapy.5,6 Enrichment of the stem cell subpopulation has been observed in glioma after irradiation.7 Thus, CSCs could be the main targets in novel cancer therapies. Creating radio- or chemosensitizers that preferentially sensitize CSCs could be a new strategy in drug development.
Quantum dots (QDs) are a new class of nanomaterials with possibility for easy manipulation of surface chemistry and optical or magnetic properties, and are thus suited for targeted anticancer therapies. QDs are about 10–100 times brighter and 100–1000 times more stable against photo-bleaching than organic dyes or fluorescent proteins, and the optical characteristics of a QD can be tuned by adjusting its size.8 It has been reported that QDs can be used for imaging of cancer cells ex vivo and for detection of mesenchymal stem cells.9,10 The QD surface can be manipulated by either directly conjugating them with antibodies and oligonucleotide probes or indirect linking by strategies such as streptavidin– biotin interaction.11 Antibody-conjugated QDs allow specific recognition and tracking of plasma membrane antigens. Snyder et al12 have used QD-CD44 conjugates for CSC detection ex vivo. However, it is not known how QDs accumulate and influence the expression of CSC surface antigens.
In this study, we investigated the effect of QDs on the expression of two putative stem cell markers, plasma membrane-associated glycoproteins CD44 and CD133, by flow cytometry and fluorescence-activated cell sorting (FACS) techniques.
Material and methods
Materials
A pancreatic adenocarcinoma (Mia-PaCa-2) cell line was purchased from the Health Protection Agency Culture Collections. Prostate adenocarcinoma (PC3) and glioblastoma (U87) cells were obtained from the American Type Culture Collection. Melanoma (FEMX-I) cells were originally derived from lymph node metastasis of a patient with melanoma at the Norwegian Radiumhospital in 1980.13 CdTe QDs coated with mercaptopropionic acid (MPA) were purchased from PlasmaChem GmbH (Berlin, Germany) emitting far-red fluorescence (λmax = 710 ± 5 nm).
Cell culturing
Mia-PaCa-2 and U87 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM). PC3 and FEMX-I cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium. Both growth mediums contained 10% fetal bovine serum (FBS, Fisher Scientific, Oslo, Norway), 100 U/mL penicillin, 100 mg/mL streptomycin, and 2 mM L-Glutamine (Sigma-Aldrich Norway AS, Oslo, Norway). In the case of the U87 cells, the DMEM medium was additionally supplemented with 3% MEM nonessential amino acids (Sigma-Aldrich). The cells were routinely cultivated in 25 cm2 Nunclon culture dishes (VWR International, Oslo, Norway) under standard conditions and were subcultured twice a week and kept in a humidified incubator at 37°C with 5% CO2. For experiments, the cells were harvested by bringing them into suspension via trypsinization and by seeding a necessary amount of cells into Nunclon multiplates.
QD staining
A stock solution of QDs was prepared by dissolving 1 mg QDs in 1 mL phosphate buffered saline (PBS) (Sigma- Aldrich) at pH 7.4 or saline (0.9% w/v NaCl). The stock solution was further diluted to a desired concentration in the growth medium and poured over the cells growing in the multiplates. The medium without QDs was poured in a few wells to have control cells in the same multiplate. After incubation at 37°C for 24 hours, the cells were washed with ice-cold PBS+ (standard PBS supplemented with 0.9 mM CaCl2 and 0.5 mM MgCl2 to prevent cell detachment at this step) to remove any free QDs. Immediately after washing, the cells were brought into suspension by trypsinization for further analysis using monoclonal antibodies.
Radiation treatment
Ionizing radiation was used as a positive treatment control with changes in the phenotype of cancer cells. FEMX-I cells were exposed to different doses ranging from 0 Gy to 10 Gy delivered by a Müller X-ray apparatus operated at 220 kV, 20 mA, with a 0.5 mm Cu filter.
Immunostaining and FACS
Up to 106 cells were labeled in PBS solution with mouse antihuman monoclonal CD44 antibody conjugated with fluorescein isothiocyanate (FITC, BD Biosciences, Franklin Lakes, NJ) and mouse antihuman monoclonal CD133 antibody conjugated with phycoerythrin (PE, eBioscience, San Diego, CA) for 20 minutes at room temperature. Respective mouse immunoglobulin and dye conjugates, IgG2b-FITC (Dako, Glostrup, Denmark) and IgG1-PE (BD Biosciences), were used as unspecific binding controls. Flow cytometric analysis was carried out with a FACSort or LSR II analyzer (BD Biosciences). The data were analyzed with FlowJo (Tree Star Inc, Ashland, OR) software. A minimum of 10,000 viable cells were measured per sample. Using forward and side scatter profiles and propidium iodide staining, debris, cell doublets, and dead cells were gated out, respectively. FACS was performed on a BD FACSAria or FACSort Cell Sorter (BD Biosciences). Purities of the sorted populations, as determined by postsorting flow analysis, were generally >90%.
Cytotoxicity assay
The cytotoxicity of the QDs was determined using a methylene blue assay on the FEMX-I cell line. The same amount of cells in each well were seeded in a 96-well plate and incubated with different concentrations of the QDs. After 24 hours of incubation the old medium was carefully aspirated, and the cells were supplemented with fresh medium and left for further growth under standard culture conditions. After 3 days the cells were washed with ice-cold PBS and fixed with 70% ethanol. After 10 minutes, 100 μL of filtered 1% w/v methylene blue aqueous solution was added to each well. After 20 minutes, the excess dye was removed by washing the wells with distilled water. To elute the dye, 200 μL of 0.2% Triton X-100 solution (Sigma-Aldrich) was added to each well and left overnight at 37°C. The plates were then gently shaken, and the absorbance at 650 nm was measured for each well with a microplate photometer (BioTek Instruments, Winooski, VT). The photometer was blanked on the first column of the wells containing the elution solvent alone. For the determination of the FEMX-I cell viability following ionizing radiation, the cells were grown for 24 hours after irradiation, and cell suspensions were analyzed by a Beckman Coulter viability analyzer (Vi-Cell XR, Beckman Coulter Inc, Brea, CA).
Results
Heterogeneity of four cancer cell lines using putative stem cell markers for CD133 and CD44 was investigated. The cell surface-associated CD44 is an adhesion glycoprotein. The function of CD133 is still unknown, but it has been shown to be involved in the dynamics of membrane protrusions and to mark CSCs. The flow cytometry technique was performed to detect fluorescence from single cells after combined staining with antibody-dye conjugates against CD133 and CD44. All cell lines expressed CD44 to a similar degree, from 96.2% to 99.9% (Figure 1). However, the expression of CD133 was very low among these cell lines (0.001%–0.23%) with slightly higher prevalence in U87 cells (1.94%, Figure 1B). Only FEMX-I cells exhibited high levels of CD133 (99.1%, Figure 1D).
The response to CD markers was not enough to determine heterogeneity of the cell lines. Therefore, we further analyzed the shape of their phenotypical patterns (Figure 1). U87 (Figure 1B) cells show nearly uniform semihorizontal oval-shaped cloud distribution. PC3 ( Figure 1A) and Mia-PaCa-2 (Figure 1C) cells show two clouds, indicating two subpopulations: circular-shaped dense cloud with high CD44 expression (CD44high) and a horizontal oval cloud with low/no CD44 expression (CD44low/−). FEMX-I cells appear to consist of three different subpopulations: CD44highCD133high, CD44highCD133low/−, and CD44low/−CD133low/− (Figure 1D).
Because FEMX-I cells showed high expression of both CD markers with three distinct subpopulations, we further examined their capability of growing and restoring initial population. Each subpopulation (CD44highCD133high, CD44highCD133low/−, and CD44low/−CD133low/−) was FACS sorted, isolated, and plated under usual growing conditions. CD44low/−CD133low/− cells did not grow after sorting. CD44highCD133high cells grew after sorting, but after 10 days of growth the cells did not show significant changes in their phenotypical composition. Immediately after sorting, the subpopulation was composed of <95% CD44highCD133high cells and remained unchanged after 10 days of growth ( Figure 2B), suggesting that CD44highCD133high subpopulation was unable to restore its progeny. However, CD44highCD133low/− cells were able to grow and change their phenotypical composition. Immediately after sorting, the cells were composed of <80% CD44highCD133low/− cells, and after 10 days of growth this subpopulation was composed of <58% CD44highCD133low/− and <41% CD44highCD133high cells, thus approaching the phenotypical composition of the unsorted cells (Figure 2C).
To determine treatment effects, first, the survival rate was measured for the FEMX-I cells. The cell viability follows a dose-response relationship: The proliferation decreases with increasing radiation dose or QD concentration (Figure 3). Second, phenotypic responses were measured following these treatments.
It is important to ensure that changes in phenotypical composition are due to the treatment and are not affected by wrong gating or background signals. After the radiation treatment, morphologic alterations in the cells can be seen, such as increased size and roughness (Figure 4A). Cell auto-fluorescence signal also alters after the treatment. The cells show a higher autofluorescence signal on the detector corresponding to CD44-FITC (Figure 4B), which must be included as a background signal when gating. The same is true for the detector corresponding to CD133-PE. The addition of the surface membrane markers shows changes in phenotypical composition caused by the treatment (Figure 4C).
The doses (7 Gy and 10 Gy) of ionizing radiation were chosen to induce death of more than half of the cells (Figure 3). The percentage of the CD44highCD133high subpopulation decreased from 72% in the control unirradiated cells to 58%–62% after irradiation, whereas that of the subpopulation CD44highCD133low/− increased from 26% to 35%–36%. The percentage of CD44lowCD133low/− subpopulation increased from 0.6% in the untreated cells to <2.4%–4.4% in the cells after irradiation (Figure 4D–F).
To investigate the response of each subpopulation to the QDs, a 50 μg/mL concentration of the CdTe- MPA QDs was chosen to be high enough to induce death of approximately half of the cells (Figure 3). A doubled concentration of the QDs was also used to observe any concentration-dependent processes. The intensity histogram of the infrared detector confirms the accumulation of the QDs in the cells (Figure 5A and B). The number of QD positives was calculated as a percentage of CD44highCD133high or CD44highCD133low/− cells (Figure 5C). Approximately 94%–99% cells in each subpopulation exhibited QD fluorescence showing no difference in the QD uptake by these subpopulations.
After the treatment with the QDs, morphologic alterations in the cells could be seen just like after the ionizing radiation: increased cell size, roughness (Figure 6A), and cell autofluorescence (Figure 6B). Again, just like for the cells treated with the ionizing radiation, the correct gating for the background signals was performed (Figure 6C). The QD treatment resulted in the appearance of a denser cloud of the cell debris (located in the left lower corner in Figure 6A), confirming that the QDs are cytotoxic to the cells. When compared with the control untreated cells, the cells treated with the lower concentration of the QDs contained 1.4 times more of the CD44highCD133low/− cells (39% versus 28% in the control) and 1.3 times less of the CD44highCD133high cells (54% versus 72% in the control) (Figure 6D and E).
The percentage of CD44low/− CD133low/− cells increased after the treatment, which might be associated with QDinduced early apoptosis. Early apoptotic cells are difficult to exclude during dead cell staining with propidium iodide; therefore, apoptotic cells might be present in this flow cytometry plot. The phenotypical composition observed in the cells treated with the higher concentration of the QDs (Figure 6F) was analogous to that of the lower concentration of the QDs. This indicates that concentration-dependent changes in the phenotypical composition were not observed.
Discussion
The property of resistance to radiation therapy is one major clinical criterion to characterize CSCs. The fraction of CSCs in a tumor could be a factor to predict resistance to the therapy.5–7 McCord et al14 have studied responsiveness to radiation in a series of glioma cell lines. Interestingly, the stem cells were more sensitive to radiation than established cell lines. The authors have concluded that a major determinant of radiosensitivity is the repair of DNA lesions, which depends on the tumor origin from which the cells were isolated. The mechanisms of CSC responsiveness to existing therapies are not yet well understood.
In this study, we have investigated population heterogeneity of prostate and pancreatic adenocarcinoma, glioblastoma, and melanoma cell lines using putative CSC markers, CD44, an adhesion molecule, and CD133, which has recently been considered as a common marker of CSCs.15 All studied cell lines exhibited high expression of CD44, whereas CD133 was highly expressed only in FEMX-I cells. Therefore, the latter cells were chosen for further examination of their stem-like properties.
Our experiments demonstrate that FEMX-I cells consist of three distinct subpopulations with different CD133 and CD44 expression levels: CD44highCD133high, CD44highCD133low/−, and CD44low/− CD133low/−. Hill16 and Rappa et al17 have also shown CD133 expression in FEMX-I cells and stated that downregulation of CD133 in FEMX-I cells results in decreased growth rate and migratory capacity, which suggests CD133 to be essential in tumor formation and regression. Interestingly, we can suggest that the subpopulation with lower expression of CD133 antigen represents tumorigenic stem cells, because only CD44highCD133low/− cells were capable of progeny restoration after their isolation and were also more resistant to the radiation treatment and the toxic effect of CdTe-MPA QDs. After the radiation or QD treatment, the FEMX-I cells maintained a higher fraction of CD44highCD133low/− cells (35%–40%) compared with that (26%–28%) in the control untreated cells.
The toxicity of CdTe QDs is associated with release of Cd2+ ions and generation of reactive oxygen species products.18 The concentration of the QDs was chosen to correspond to lethal concentrations of LC40 and LC30, ie, high enough to induce obvious QD-associated cell death and thus to examine whether a QD-resistant subpopulation exists. Although no significant differences in CdTe-MPA accumulation among CD44highCD133high, CD44highCD133low/−, and CD44low/−CD133low/− subpopulations were observed, these subpopulations showed different behavior. The percentage of CD44highCD133low/− cells actually increased after the QD treatment.
The CD133 was the first identified membrane protein named prominin for its prominent location on the protrusion of cell membranes.15 Rappa et al17 have suggested that the CD133 can be a potential target for antimelanoma therapy. In our study, we have divided the CD133+ population into two subpopulations, CD133high and CD133low/−, which showed different responses to the therapy.
When considering novel targeted therapies it is important to know whether a new therapeutic agent can actually accumulate in a cell. In this study we show that, although the FEMX-I cell line is heterogeneous, the CdTe-MPA QDs accumulate equally in different cell subpopulations. To our knowledge, this is the first time QD accumulation in different subpopulations of cancer stem-like cells has been evaluated.
The other important aspect is to ensure that the therapeutic agent binds to the desired target. Lidke et al19 targeted cancer cells by conjugating QDs to a human epidermal growth factor, a small protein with a specific affinity to its membrane receptor. Fluorescence emitted from such QDs allowed continuous observation of the cells. Wang et al20 demonstrated a CSC targeting therapy. Photothermolysis with carbon nanotubes conjugated with anti-CD133 antibody selectively eradicated CD133 positive glioma cells. Current interest in QDs lies in their attractive imaging properties. QDs conjugated with a targeting antibody also have the potential to label and eliminate desired subpopulations of cancer cells.
Conclusion
Our experiments show that the FEMX-I cell line is heterogeneous and can be divided into subpopulations with higher and lower expressions of CD44 and CD133. The subpopulation with the lower expression of CD133 (CD44highCD133low/−) displays characteristics of CSCs. It is more resistant to ionizing radiation or QD cytotoxicity and able to restore its progeny. There is no difference in the accumulation of the CdTe-MPA QDs among different FEMX-I subpopulations. These results encourage further investigations on the applicability of QDs in CSC targeting therapies.
Acknowledgments
This study was supported by the Research Council of Lithuania Grant No. LIG-15/2010, the EU and Norwegian Grant No. 2004-LT0036-IP-1 NOR. The work of Simona (Petrosiute) Steponkiene was funded by the European Commission’s ERASMUS Lifelong Learning Programme (LLP). Petras Juzenas and Simona (Lukoseviciute) Kavaliauskiene were supported by the Research Council of Norway (Grant No. 182058), the South-Eastern Norway Regional Health Authority (Grant No. 20100068), and the Norwegian Radium Hospital Research Foundation (Grant No. FU0802). We are grateful to Dr Lina Prasmickaite for helpful advice. The Flow Cytometry Core Facility at the Radiumhospital, by Idun Dale Rein, is acknowledged for help with analyses and sorting.
Footnotes
Disclosure
The authors report no conflicts of interest in this work.
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