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. 1982 Feb 25;10(4):1335–1343. doi: 10.1093/nar/10.4.1335

The complete sequence of RNA segment 2 of influenza A/NT/60/68 P1 protein.

D H Bishop, J A Huddleston, G G Brownlee
PMCID: PMC320529  PMID: 7041090

Abstract

A DNA copy of influenza A/NT/60/68 viral RNA segment 2, corresponding to protein P1, has been cloned in the E.coli plasmid pBr322. The clone is 2341 nucleotides long and represents a full-length copy of the viral RNA. In the viral complementary (plus sense) strand there is an open reading frame that is 2271 nucleotides long. The predicted primary gene product is a basic 86,300 dalton protein with a net charge at neutral pH of +23. A 29 amino acid stretch of the protein (coded by nucleotide residues 583-669) is highly basic and contains 7 lysine and 8 arginine residues. Other smaller clusters of basic amino acids are also present in the protein.

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Selected References

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