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. 1982 Mar 11;10(5):1653–1659. doi: 10.1093/nar/10.5.1653

The nucleotide sequence of the maize and spinach chloroplast isoleucine transfer RNA encoded in the 16S to 23S rDNA spacer.

P Guillemaut, J H Weil
PMCID: PMC320556  PMID: 6917975

Abstract

The sequence of maize chloroplast tRNAIle2, encoded in the 16S to 23S rDNA spacer, was determined using in vitro labeling techniques. The sequence is: pG-G-G-C-U-A-U-U-A-G-C-U-C-A-G-U-Gm-G-D-A-G-A-G-C-m22G-C-G-C-C-C-C-U-G-A-U-t6A- A-G-G-G-C-G-A-G-m7G-acp3U-C-U-C-U-G-G-T-psi-C-A-A-G-U-C-C-A-G-G-A-U-G-G-C-C-C-A -C-C-AOH. This sequence is identical to that predicted from the corresponding gene sequence, after excision of a long intervening sequence (1), but shows the post-transcriptional modifications of this tRNA. Furthermore it demonstrates that the excision of the intron occurs after the second base following the anticodon and that this gene, which is over 1000 base-pair long, is transcribed and processed into a mature functional chloroplast-tRNA. The sequence of maize (a monocot) and spinach (a dicot) tRNAIle2 are shown to be identical.

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Selected References

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  1. Bohnert H. J., Driesel A. J., Crouse E. J., Gordon K., Herrmann R. G., Steinmetz A., Mubumbila M., Keller M., Burkard G., Weil J. H. Presence of a transfer RNA gene in the spacer sequence between the 16 S and 23 S rRNA genes of spinach chloroplast DNA. FEBS Lett. 1979 Jul 1;103(1):52–56. doi: 10.1016/0014-5793(79)81248-x. [DOI] [PubMed] [Google Scholar]
  2. Calagan J. L., Pirtle R. M., Pirtle I. L., Kashdan M. A., Vreman H. J., Dudock B. S. Homology between chloroplast and prokaryotic initiator tRNA. Nucleotide sequence of spinach chloroplast methionine initiator tRNA. J Biol Chem. 1980 Oct 25;255(20):9981–9984. [PubMed] [Google Scholar]
  3. Canaday J., Guillemaut P., Gloeckler R., Weil J. H. The nucleotide sequence of spinach chloroplast tryptophan transfer RNA. Nucleic Acids Res. 1981 Jan 10;9(1):47–53. doi: 10.1093/nar/9.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Canaday J., Guillemaut P., Weil J. H. The nucleotide sequences of the initiator transfer RNAs from bean cytoplasm and chloroplasts. Nucleic Acids Res. 1980 Mar 11;8(5):999–1008. doi: 10.1093/nar/8.5.999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chang S. H., Brum C. K., Siberklang M., RajBhandary U. L., Hecker L. I., Barnett W. E. The first nucleotide sequence of an organelle transfer RNA: chloroplastic tRNAphe. Cell. 1976 Dec;9(4 Pt 2):717–723. doi: 10.1016/0092-8674(76)90135-5. [DOI] [PubMed] [Google Scholar]
  6. Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Driesel A. J., Crouse E. J., Gordon K., Bohnert H. J., Herrmann R. G., Steinmetz A., Mubumbila M., Keller M., Burkard G., Weil J. H. Fractionation and identification of spinach chloroplast transfer RNAs and mapping of their genes on the restriction map of chloroplast DNA. Gene. 1979 Aug;6(4):285–306. doi: 10.1016/0378-1119(79)90070-2. [DOI] [PubMed] [Google Scholar]
  8. Fradin A., Gruhl H., Feldmann H. Mapping of yeast tRNAs by two-dimensional electrophoresis on polyacrylamide gels. FEBS Lett. 1975 Feb 1;50(2):185–189. doi: 10.1016/0014-5793(75)80485-6. [DOI] [PubMed] [Google Scholar]
  9. Graf L., Kössel H., Stutz E. Sequencing of 16S--23S spacer in a ribosomal RNA operon of Euglena gracilis chloroplast DNA reveals two tRNA genes. Nature. 1980 Aug 28;286(5776):908–910. doi: 10.1038/286908a0. [DOI] [PubMed] [Google Scholar]
  10. Guillemaut P., Keith G. Primary structure of bean chloroplastic tRNAPhe. Comparison with Euglena chloroplastic tRNAPhe. FEBS Lett. 1977 Dec 15;84(2):351–356. doi: 10.1016/0014-5793(77)80723-0. [DOI] [PubMed] [Google Scholar]
  11. Heckman J. E., Alzner-Deweerd B., RajBhandary U. L. Interesting and unusual features in the sequence of Neurospora crassa mitochondrial tyrosine transfer RNA. Proc Natl Acad Sci U S A. 1979 Feb;76(2):717–721. doi: 10.1073/pnas.76.2.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ikemura T., Nomura M. Expression of spacer tRNA genes in ribosomal RNA transcription units carried by hybrid Col E1 plasmids in E. coli. Cell. 1977 Aug;11(4):779–793. doi: 10.1016/0092-8674(77)90291-4. [DOI] [PubMed] [Google Scholar]
  13. Kashdan M. A., Pirtle R. M., Pirtle I. L., Calagan J. L., Vreman H. J., Dudock B. S. Nucleotide sequence of a spinach chloroplast threonine tRNA. J Biol Chem. 1980 Sep 25;255(18):8831–8835. [PubMed] [Google Scholar]
  14. Keller M., Burkard G., Bohnert H. J., Mubumbila M., Gordon K., Steinmetz A., Heiser D., Crouse E. J., Weil J. H. Transfer RNA genes associated with the 16S and 23S rRNA genes of Euglena chloroplast DNA. Biochem Biophys Res Commun. 1980 Jul 16;95(1):47–54. doi: 10.1016/0006-291x(80)90702-0. [DOI] [PubMed] [Google Scholar]
  15. Kenerley M. E., Morgan E. A., Post L., Lindahl L., Nomura M. Characterization of hybrid plasmids carrying individual ribosomal ribonucleic acid transcription units of Escherichia coli. J Bacteriol. 1977 Dec;132(3):931–949. doi: 10.1128/jb.132.3.931-949.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kiss A., Sain B., Venetianer P. The number of rRNA genes in Escherichia coli. FEBS Lett. 1977 Jul 1;79(1):77–79. doi: 10.1016/0014-5793(77)80354-2. [DOI] [PubMed] [Google Scholar]
  17. Knapp G., Ogden R. C., Peebles C. L., Abelson J. Splicing of yeast tRNA precursors: structure of the reaction intermediates. Cell. 1979 Sep;18(1):37–45. doi: 10.1016/0092-8674(79)90351-9. [DOI] [PubMed] [Google Scholar]
  18. Koch W., Edwards K., Kössel H. Sequencing of the 16S-23S spacer in a ribosomal RNA operon of Zea mays chloroplast DNA reveals two split tRNA genes. Cell. 1981 Jul;25(1):203–213. doi: 10.1016/0092-8674(81)90245-2. [DOI] [PubMed] [Google Scholar]
  19. Lund E., Dahlberg J. E., Lindahl L., Jaskunas S. R., Dennis P. P., Nomura M. Transfer RNA genes between 16S and 23S rRNA genes in rRNA transcription units of E. coli. Cell. 1976 Feb;7(2):165–177. doi: 10.1016/0092-8674(76)90016-7. [DOI] [PubMed] [Google Scholar]
  20. Malnoë P., Rochaix J. D. Localization of 4S RNA genes on the chloroplast genome of Chlamydomonas reinhardii. Mol Gen Genet. 1978 Nov 9;166(3):269–275. doi: 10.1007/BF00267618. [DOI] [PubMed] [Google Scholar]
  21. Martin R. P., Sibler A. P., Schneller J. M., Keith G., Stahl A. J., Dirheimer G. Primary structure of yeast mitochondrial DNA-coded phenylalanine-tRNA. Nucleic Acids Res. 1978 Dec;5(12):4579–4592. doi: 10.1093/nar/5.12.4579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. McCoy J. M., Jones D. S. The nucleotide sequence of Scenedesmus obliquus chloroplast tRNAfMet. Nucleic Acids Res. 1980 Nov 11;8(21):5089–5093. doi: 10.1093/nar/8.21.5089. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Morgan E. A., Ikemura T., Lindahl L., Fallon A. M., Nomura M. Some rRNA operons in E. coli have tRNA genes at their distal ends. Cell. 1978 Feb;13(2):335–344. doi: 10.1016/0092-8674(78)90202-7. [DOI] [PubMed] [Google Scholar]
  24. Orozco E. M., Jr, Gray P. W., Hallick R. B. Euglena gracilis chloroplast ribosomal RNA transcription units. I. The location of transfer RNA, 5 S, 16 S, and 23 S ribosomal RNA genes. J Biol Chem. 1980 Nov 25;255(22):10991–10996. [PubMed] [Google Scholar]
  25. Orozco E. M., Jr, Rushlow K. E., Dodd J. R., Hallick R. B. Euglena gracilis chloroplast ribosomal RNA transcription units. II. Nucleotide sequence homology between the 16 S--23 S ribosomal RNA spacer and the 16 S ribosomal RNA leader regions. J Biol Chem. 1980 Nov 25;255(22):10997–11003. [PubMed] [Google Scholar]
  26. Pirtle R., Calagan J., Pirtle I., Kashdan M., Vreman H., Dudock B. The nucleotide sequence of spinach chloroplast methionine elongator tRNA. Nucleic Acids Res. 1981 Jan 10;9(1):183–188. doi: 10.1093/nar/9.1.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sanger F., Coulson A. R. The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978 Mar 1;87(1):107–110. doi: 10.1016/0014-5793(78)80145-8. [DOI] [PubMed] [Google Scholar]
  28. Sekiya T., Nishimura S. Sequence of the gene for isoleucine tRNA1 and the surrounding region in a ribosomal RNA operon of Escherichia coli. Nucleic Acids Res. 1979 Feb;6(2):575–592. doi: 10.1093/nar/6.2.575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Silberklang M., Gillum A. M., RajBhandary U. L. The use of nuclease P1 in sequence analysis of end group labeled RNA. Nucleic Acids Res. 1977 Dec;4(12):4091–4108. doi: 10.1093/nar/4.12.4091. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Sprouse H. M., Kashdan M., Otis L., Dudock B. Nucleotide sequence of a spinach chloroplast valine tRNA. Nucleic Acids Res. 1981 Jun 11;9(11):2543–2547. doi: 10.1093/nar/9.11.2543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Stanley J., Vassilenko S. A different approach to RNA sequencing. Nature. 1978 Jul 6;274(5666):87–89. doi: 10.1038/274087a0. [DOI] [PubMed] [Google Scholar]
  32. Young R. A., Macklis R., Steitz J. A. Sequence of the 16 S-23 s spacer region in two ribosomal RNA operons of Escherichia coli. J Biol Chem. 1979 May 10;254(9):3264–3271. [PubMed] [Google Scholar]

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