Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1982 Mar 11;10(5):1679–1689. doi: 10.1093/nar/10.5.1679

T-DNA of pTi-15955 from Agrobacterium tumefaciens is transcribed into a minimum of seven polyadenylated RNAs in a sunflower crown gall tumor.

N Murai, J D Kemp
PMCID: PMC320558  PMID: 6175956

Abstract

Northern blot hybridization analysis of polysomal polyadenylated RNA isolated from sunflower crown gall tumor PSCG-15955 demonstrated that a minimum of seven RNAs were transcribed from T-DNA of pTi-15955 from Agrobacterium tumefaciens. The sizes of the T-DNA transcripts were 1.8, 1.6, 1.5, 1.1, 1.0 kilo bases (kb) and two transcripts of 0.8 kb long. The relative abundance of these polyadenylated RNAs varied greatly, the 1.0 kb RNA being the most abundant and the 1.6 kb RNA being the least abundant. Assignment of map locations of the seven polyadenylated RNAs indicated that the conserved region of T-DNA which may play a central role in tumorigenesis contained four RNAs of 1.8, 1.1, 0.8(a) and a portion of 0.8(b) kb long.

Full text

PDF
1679

Images in this article

1684Image
on p.1684
1685Image
on p.1685
1686Image
on p.1686

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRAUN A. C. The activation of two growth-substance systems accompanying the conversion of normal to tumor cells in crown gall. Cancer Res. 1956 Jan;16(1):53–56. [PubMed] [Google Scholar]
  2. Bantle J. A., Maxwell I. H., Hahn W. E. Specificity of oligo (dT)-cellulose chromatography in the isolation of polyadenylated RNA. Anal Biochem. 1976 May 7;72:413–427. doi: 10.1016/0003-2697(76)90549-2. [DOI] [PubMed] [Google Scholar]
  3. Chilton M. D., Drummond M. H., Merio D. J., Sciaky D., Montoya A. L., Gordon M. P., Nester E. W. Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell. 1977 Jun;11(2):263–271. doi: 10.1016/0092-8674(77)90043-5. [DOI] [PubMed] [Google Scholar]
  4. Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Currier T. C., Nester E. W. Evidence for diverse types of large plasmids in tumor-inducing strains of Agrobacterium. J Bacteriol. 1976 Apr;126(1):157–165. doi: 10.1128/jb.126.1.157-165.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ditta G., Stanfield S., Corbin D., Helinski D. R. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347–7351. doi: 10.1073/pnas.77.12.7347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Garfinkel D. J., Nester E. W. Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism. J Bacteriol. 1980 Nov;144(2):732–743. doi: 10.1128/jb.144.2.732-743.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gelvin S. B., Gordon M. P., Nester E. W., Aronson A. I. Transcription of the Agrobacterium Ti plasmid in the bacterium and in crown gall tumors. Plasmid. 1981 Jul;6(1):17–29. doi: 10.1016/0147-619x(81)90051-2. [DOI] [PubMed] [Google Scholar]
  9. Girvitz S. C., Bacchetti S., Rainbow A. J., Graham F. L. A rapid and efficient procedure for the purification of DNA from agarose gels. Anal Biochem. 1980 Aug;106(2):492–496. doi: 10.1016/0003-2697(80)90553-9. [DOI] [PubMed] [Google Scholar]
  10. Gurley W. B., Kemp J. D., Albert M. J., Sutton D. W., Callis J. Transcription of Ti plasmid-derived sequences in three octopine-type crown gall tumor lines. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2828–2832. doi: 10.1073/pnas.76.6.2828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Humphreys G. O., Willshaw G. A., Anderson E. S. A simple method for the preparation of large quantities of pure plasmid DNA. Biochim Biophys Acta. 1975 Apr 2;383(4):457–463. doi: 10.1016/0005-2787(75)90318-4. [DOI] [PubMed] [Google Scholar]
  12. MENAGE A., MOREL G. SUR LA PR'ESENCE D'OCTOPINE DANS LES TISSUS DE CROWN-GALL. C R Hebd Seances Acad Sci. 1964 Dec 21;259:4795–4796. [PubMed] [Google Scholar]
  13. Matzke A. J., Chilton M. D. Site-specific insertion of genes into T-DNA of the Agrobacterium tumor-inducing plasmid: an approach to genetic engineering of higher plant cells. J Mol Appl Genet. 1981;1(1):39–49. [PubMed] [Google Scholar]
  14. McMaster G. K., Carmichael G. G. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835–4838. doi: 10.1073/pnas.74.11.4835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Merlo D. J., Kemp J. D. Attempts to Detect Agrobacterium tumefaciens DNA in Crown-Gall Tumor Tissue. Plant Physiol. 1976 Jul;58(1):100–106. doi: 10.1104/pp.58.1.100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ooms G., Klapwijk P. M., Poulis J. A., Schilperoort R. A. Characterization of Tn904 insertions in octopine Ti plasmid mutants of Agrobacterium tumefaciens. J Bacteriol. 1980 Oct;144(1):82–91. doi: 10.1128/jb.144.1.82-91.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sciaky D., Montoya A. L., Chilton M. D. Fingerprints of Agrobacterium Ti plasmids. Plasmid. 1978 Feb;1(2):238–253. doi: 10.1016/0147-619x(78)90042-2. [DOI] [PubMed] [Google Scholar]
  18. Smith E. F., Townsend C. O. A PLANT-TUMOR OF BACTERIAL ORIGIN. Science. 1907 Apr 26;25(643):671–673. doi: 10.1126/science.25.643.671. [DOI] [PubMed] [Google Scholar]
  19. Sun S. M., Buchbinder B. U., Hall T. C. Cell-free Synthesis of the Major Storage Protein of the Bean, Phaseolus vulgaris L. Plant Physiol. 1975 Dec;56(6):780–785. doi: 10.1104/pp.56.6.780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Thomashow M. F., Nutter R., Montoya A. L., Gordon M. P., Nester E. W. Integration and organization of Ti plasmid sequences in crown gall tumors. Cell. 1980 Mar;19(3):729–739. doi: 10.1016/s0092-8674(80)80049-3. [DOI] [PubMed] [Google Scholar]
  22. Thomashow M. F., Nutter R., Postle K., Chilton M. D., Blattner F. R., Powell A., Gordon M. P., Nester E. W. Recombination between higher plant DNA and the Ti plasmid of Agrobacterium tumefaciens. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6448–6452. doi: 10.1073/pnas.77.11.6448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Vogelstein B., Gillespie D. Preparative and analytical purification of DNA from agarose. Proc Natl Acad Sci U S A. 1979 Feb;76(2):615–619. doi: 10.1073/pnas.76.2.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Willmitzer L., Otten L., Simons G., Schmalenbach W., Schröder J., Schröder G., Van Montagu M., de Vos G., Schell J. Nuclear and polysomal transcripts of T-DNA in octopine crown gall suspension and callus cultures. Mol Gen Genet. 1981;182(2):255–262. doi: 10.1007/BF00269667. [DOI] [PubMed] [Google Scholar]
  26. Zaenen I., Van Larebeke N., Van Montagu M., Schell J. Supercoiled circular DNA in crown-gall inducing Agrobacterium strains. J Mol Biol. 1974 Jun 15;86(1):109–127. doi: 10.1016/s0022-2836(74)80011-2. [DOI] [PubMed] [Google Scholar]
  27. Zambryski P., Holsters M., Kruger K., Depicker A., Schell J., Van Montagu M., Goodman H. M. Tumor DNA structure in plant cells transformed by A. tumefaciens. Science. 1980 Sep 19;209(4463):1385–1391. doi: 10.1126/science.6251546. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES