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. 1982 Apr 10;10(7):2337–2351. doi: 10.1093/nar/10.7.2337

A general method for the synthesis of 5'-monophosphates of DNA fragments via phosphotriester intermediates.

G A van der Marel, C A van Boeckel, G Wille, J H van Boom
PMCID: PMC320613  PMID: 7088712

Abstract

A new and attractive phosphorylation procedure which allows the introduction, via phosphotriester intermediates, of 5'-phosphate functions of DNA fragments is described. The method is based on the activation of bifunctional phosphorylating agents with 1-hydroxybenzotriazole. The approach will be exemplified by the synthesis of pACGC using four different 5'-phosphotriester intermediates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. van Boom J. H., de Rooy J. F. Sequence analysis of synthetic oligonucleotides by high-performance liquid anion-exchange chromatography. J Chromatogr. 1977 Jan 21;131:169–177. doi: 10.1016/s0021-9673(00)80930-9. [DOI] [PubMed] [Google Scholar]

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