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. 1982 Apr 10;10(7):2399–2414. doi: 10.1093/nar/10.7.2399

Laser crosslinking of E. coli RNA polymerase and T7 DNA.

C A Harrison, D H Turner, D C Hinkle
PMCID: PMC320618  PMID: 7045809

Abstract

The first photochemical crosslinking of a protein to a nucleic acid using laser excitation is reported. A single, 120 mJ, 20 ns pulse at 248 nm crosslinks about 10% of bound E. coli RNA polymerase to T7 DNA under the conditions studied. The crosslinking yield depends on mercaptoethanol concentration, and is a linear function of laser intensity. The protein subunits crosslinked to DNA are beta, beta' and sigma.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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