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. Author manuscript; available in PMC: 2012 Nov 24.
Published in final edited form as: Neuroscience. 2011 Sep 10;196:80–96. doi: 10.1016/j.neuroscience.2011.09.004

Figure 1.

Figure 1

Representative images used to guide quantification of brain regions of interest and processed for in situ hybridization. A) Acetylcholinesterase staining was performed on sections adjacent to those processed for c-fos mRNA and served as a guide to identify nuclei of the amygdala. B) Tissue processed for vglut-2 mRNA to identify thalamic and habenular nuclei. C) Representative image of tissue processed for c-fos mRNA using in situ hybridization.