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. 1982 Apr 24;10(8):2597–2608. doi: 10.1093/nar/10.8.2597

Studies on deoxyribonucleic acids and related compounds. V. Synthesis of pentadecanucleotide duplex containing the ideal Pribnow sequence of promoter by the phosphotriester method using a new phosphorylating reagent.

E Ohtsuka, Y Taniyama, R Marumoto, H Sato, H Hirosaki, M Ikehara
PMCID: PMC320636  PMID: 7079181

Abstract

A phosphorylating reagent o-chlorophenyl phosphoro-p-anisi-dochloridate was synthesized to phosphorylate the 3'-hydroxyl group of N, 5'-protected deoxynucleosides. These nucleotides served as 3'-terminal units for the synthesis of oligonucleotide blocks. By condensation of these oligonucleotide blocks the partially complementary deoxypentadecanucleotides dAGCTTATAATGC-TCG and dAGCTCGAGCATTATA, which contained the ideal Pribnow sequence TATAATG, were synthesized.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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