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. 1982 May 11;10(9):2763–2773. doi: 10.1093/nar/10.9.2763

Primary and secondary structure specificity of the cleavage of 'single-stranded' DNA by endonuclease Hinf I.

B Hofer, G Ruhe, A Koch, H Köster
PMCID: PMC320654  PMID: 6285307

Abstract

The interaction of endonuclease Hinf I with single-stranded fd DNA was examined. The sizes of the cleavage products indicate that the enzyme cuts this substrate at the same sequences as double-stranded DNA (GANTC). To determine whether or not the recognition sites in single-stranded DNA have to be present in double-stranded form in order to be cleaved, DNA fragments containing complementary or non-complementary Hinf I sequences were prepared and treated as substrates. The results suggest that completely base-paired recognition sites are necessary for cleavage. Sequences surrounding the Hinf I pentanucleotides significantly modulate the reaction rates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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