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. 1982 May 25;10(10):3241–3247. doi: 10.1093/nar/10.10.3241

Nucleotide sequence of wheat germ cytoplasmic initiator methionine transfer ribonucleic acid.

H P Ghosh, K Ghosh, M Simsek, U L RajBhandary
PMCID: PMC320703  PMID: 6808465

Abstract

The primary sequence of wheat germ initiator tRNA has been determined using in vitro labelling techniques. The sequence is: pAUCAGAGUm1Gm2GCGCAG CGGAAGCGUm2GG psi GGGCCCAUt6AACCCACAGm7GDm5Cm5CCAGGA psi CGm1AAACCUG*GCUCUGAUACCAOH. As in other eukaryotic initiator tRNAs, the sequence -T psi CG(A)- present in loop IV of virtually all tRNA active in protein synthesis is absent and is replaced by -A psi CG-. The base pair G2:C71 present in all other initiator tRNAs recognized by E. coli Met-tRNA transformylase is absent and is replaced by U2:A71. Since wheat germ initiator tRNA is not formylated by E. coli Met-tRNA transformylase this implies a possible role of the G2:C71 base pair present in other initiator tRNAs in formylation of initiator tRNA species.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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