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. 1982 May 25;10(10):3249–3260. doi: 10.1093/nar/10.10.3249

Syringe method for stepwise chemical synthesis of oligonucleotides.

T Tanaka, R L Letsinger
PMCID: PMC320704  PMID: 7099961

Abstract

A simple procedure is described for synthesis of oligonucleotides by phosphate chemistry. Chains can be constructed rapidly with minimal equipment (a syringe and reagent bottles). The method is illustrated by synthesis of d-TGCAGGTT. Pertinent supporting data on the effect of variations in the detritylation, condensation, oxidation, capping and cleavage steps in the synthetic approach and in isolation procedures are also presented.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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