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. 1982 May 25;10(10):3261–3277. doi: 10.1093/nar/10.10.3261

The B reversible Z transition of poly(dI-br5dC).poly(dI-br5dC). A quantitative description of the Z form dynamic structure.

B Hartmann, J Pilet, M Ptak, J Ramstein, B Malfoy, M Leng
PMCID: PMC320705  PMID: 7099962

Abstract

The study of poly(dI-br5dC).poly(dI-br5dC) films by infrared spectroscopy shows that in low salt concentration, the conformation of this polynucleotide belongs to the B-family and in high salt concentration to the Z-family. 31P nuclear magnetic resonance and circular dichroism confirm the existence of these two forms. By circular dichroism and ultraviolet absorption, it is shown that the equilibrium constant of the B reversible Z transition depends upon temperature. The deuteration rates of exchangeable protons involved in hydrogen bonds between base pairs were deduced from the changes in absorbance near 1700 cm-1. In the B-form, one class of protons is measured with an exchange half-time of 20 minutes. In the Z-form, two classes of protons are measured with very different exchange half-times, the exchange half-time of the slow protons being of the order of 850 minutes. By comparison of these results with those previously obtained for poly(dG-dC).poly(dG-dC), these very slow protons of these two Z-polynucleotides are identified as the cytosine amino protons. A quantitative description of the dynamic structure of the Z-form is presented.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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