Abstract
A method for the synthesis of mixed dimers, trimers and oligonucleotides on a solid support using monomeric protected nucleoside phosphochloridites (1a-d) has been developed and the different nucleoside reagents, and the results show that yields of different oligomers in a mixture could be directly correlated to the concentration of the four reagents. Separation of mixed oligomers on a reversed phase C18 column has also been studied.
Full text
PDF![3291](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee2d/320707/5fc0e9a5dda9/nar00379-0260.png)
![3292](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee2d/320707/b3b31e1a98d6/nar00379-0261.png)
![3293](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee2d/320707/1e2d4b9dfc16/nar00379-0262.png)
![3294](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee2d/320707/309cdbafcf5e/nar00379-0263.png)
![3295](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee2d/320707/5cd91d0e7ad1/nar00379-0264.png)
![3296](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee2d/320707/fbe24f4a8344/nar00379-0265.png)
![3297](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee2d/320707/cefc9e30591a/nar00379-0266.png)
![3298](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee2d/320707/78650642b4ba/nar00379-0267.png)
![3299](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee2d/320707/4a8b64de0c87/nar00379-0268.png)
![3300](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee2d/320707/4824fb5c0e9d/nar00379-0269.png)
![3301](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee2d/320707/3b3d39760d13/nar00379-0270.png)
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Caruthers M. H., Beaucage S. L., Efcavitch J. W., Fisher E. F., Matteucci M. D., Stabinsky Y. New chemical methods for synthesizing polynucleotides. Nucleic Acids Symp Ser. 1980;(7):215–223. [PubMed] [Google Scholar]
- Chow F., Kempe T., Palm G. Synthesis of oligodeoxyribonucleotides on silica gel support. Nucleic Acids Res. 1981 Jun 25;9(12):2807–2817. doi: 10.1093/nar/9.12.2807. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Daub G. W., van Tamelen E. E. Synthesis of oligoribonucleotides based on the facile cleavage of methyl phosphotriester intermediates. J Am Chem Soc. 1977 May 11;99(10):3526–3528. doi: 10.1021/ja00452a069. [DOI] [PubMed] [Google Scholar]
- Gait M. J., Singh M., Sheppard R. C., Edge M. D., Greene A. R., Heathcliffe G. R., Atkinson T. C., Newton C. R., Markham A. F. Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates. Nucleic Acids Res. 1980 Mar 11;8(5):1081–1096. doi: 10.1093/nar/8.5.1081. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Letsinger R. L., Lunsford W. B. Synthesis of thymidine oligonucleotides by phosphite triester intermediates. J Am Chem Soc. 1976 Jun 9;98(12):3655–3661. doi: 10.1021/ja00428a045. [DOI] [PubMed] [Google Scholar]
- Narang S. A., Brousseau R., Hsiung H. M., Michniewicz J. J. Chemical synthesis of deoxyoligonucleotides by the modified triester method. Methods Enzymol. 1980;65(1):610–620. doi: 10.1016/s0076-6879(80)65063-0. [DOI] [PubMed] [Google Scholar]
- Tu C. P., Wu R. Sequence analysis of short DNA fragments. Methods Enzymol. 1980;65(1):620–638. [PubMed] [Google Scholar]
- Wallace R. B., Johnson M. J., Hirose T., Miyake T., Kawashima E. H., Itakura K. The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA. Nucleic Acids Res. 1981 Feb 25;9(4):879–894. doi: 10.1093/nar/9.4.879. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weber H., Khorana H. G. CIV. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast. Chemical synthesis of an icosadeoxynucleotide corresponding to the nucleotide sequence 21 to 40. J Mol Biol. 1972 Dec 28;72(2):219–249. doi: 10.1016/0022-2836(72)90147-7. [DOI] [PubMed] [Google Scholar]