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. 1982 Jun 11;10(11):3381–3389. doi: 10.1093/nar/10.11.3381

Two distinct conformations of rat liver ribosomal 5S RNA.

I Toots, R Misselwitz, S Böhm, H Welfle, R Villems, M Saarma
PMCID: PMC320718  PMID: 7099965

Abstract

Three different conformers of rat liver 5S ribosomal RNA were investigated by partial nuclease cleavage technique using S1 nuclease and cobra venom endoribonuclease (CVE) as conformational probes. Urea-treated and renatured 5S RNA co-migrate on non-denaturing gels, but exhibit distinct differences in their nuclease cleavage patterns. The most prominent differences in S1 nuclease and CVE accessibility of these conformers are located in region 30-50 and around nucleotides 70 and 90. The third form of 5S RNA with higher electrophoretic mobility was generated by EDTA treatment. The cleavage patterns of this 5S RNA conformer are similar to that characteristic for the renatured 5S RNA. The results demonstrate the difference in secondary structure and possibly different tertiary base-pairing interactions of 5S RNA conformers.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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