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. 1982 Jun 11;10(11):3495–3502. doi: 10.1093/nar/10.11.3495

Chromatin diminution in Ascaris suum: nucleotide sequence of the eliminated satellite DNA.

R E Streeck, K B Moritz, K Beer
PMCID: PMC320726  PMID: 6285304

Abstract

We have determined the prototype sequence of the DNA which is eliminated in the course of chromatin diminution in Ascaris suum. This DNA which is virtually absent from somatic cells but retained in the germ line consists predominantly of highly repetitive sequences which are variants of an AT rich 123 base pair repeat unit. Both major and minor variants have been sequenced. The overall structure of this germ line limited DNA corresponds to the segmental organization characteristic of satellite DNAs. Possible correlations between the mechanism of chromatin diminution and some properties of the satellite sequence are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bateman A. J. Letter: Simplification of palindromic telomere theory. Nature. 1975 Jan 31;253(5490):379–380. doi: 10.1038/253379a0. [DOI] [PubMed] [Google Scholar]
  2. Beams H. W., Kessel R. G. The problem of germ cell determinants. Int Rev Cytol. 1974;39:413–479. doi: 10.1016/s0074-7696(08)60944-4. [DOI] [PubMed] [Google Scholar]
  3. Beermann S., Meyer G. F. Chromatin rings as products of chromatin diminution in Cyclops. Chromosoma. 1980;77(3):277–283. doi: 10.1007/BF00286053. [DOI] [PubMed] [Google Scholar]
  4. Beermann S. The diminution of Heterochromatic chromosomal segments in Cyclops (Crustacea, Copepoda). Chromosoma. 1977 Apr 20;60(4):297–344. doi: 10.1007/BF00292858. [DOI] [PubMed] [Google Scholar]
  5. Brutlag D. L. Molecular arrangement and evolution of heterochromatic DNA. Annu Rev Genet. 1980;14:121–144. doi: 10.1146/annurev.ge.14.120180.001005. [DOI] [PubMed] [Google Scholar]
  6. Cavalier-Smith T. Palindromic base sequences and replication of eukaryote chromosome ends. Nature. 1974 Aug 9;250(5466):467–470. doi: 10.1038/250467a0. [DOI] [PubMed] [Google Scholar]
  7. Klobutcher L. A., Swanton M. T., Donini P., Prescott D. M. All gene-sized DNA molecules in four species of hypotrichs have the same terminal sequence and an unusual 3' terminus. Proc Natl Acad Sci U S A. 1981 May;78(5):3015–3019. doi: 10.1073/pnas.78.5.3015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  9. Moritz K. B., Roth G. E. Complexity of germline and somatic DNA in Ascaris. Nature. 1976 Jan 1;259(5538):55–57. doi: 10.1038/259055a0. [DOI] [PubMed] [Google Scholar]
  10. Pech M., Streeck R. E., Zachau H. G. Patchwork structure of a bovine satellite DNA. Cell. 1979 Nov;18(3):883–893. doi: 10.1016/0092-8674(79)90140-5. [DOI] [PubMed] [Google Scholar]
  11. Plucienniczak A., Streeck R. E. An alternative procedure for the strand separation of DNA fragments. FEBS Lett. 1981 Feb 9;124(1):72–74. doi: 10.1016/0014-5793(81)80056-7. [DOI] [PubMed] [Google Scholar]
  12. Roth G. E., Moritz K. B. Restriction enzyme analysis of the germ line limited DNA of Ascaris suum. Chromosoma. 1981;83(2):169–190. doi: 10.1007/BF00286787. [DOI] [PubMed] [Google Scholar]
  13. Roth G. E. Satellite DNA properties of the germ line limited DNA and the organization of the somatic genomes in the nematodes Ascaris suum and Parascaris equorum. Chromosoma. 1979 Oct 1;74(3):355–371. doi: 10.1007/BF01190750. [DOI] [PubMed] [Google Scholar]
  14. Sager R., Kitchin R. Selective silencing of eukaryotic DNA. Science. 1975 Aug 8;189(4201):426–433. [PubMed] [Google Scholar]
  15. Streeck R. E. Single-strand and double-strand cleavage at half-modified and fully modified recognition sites for the restriction nucleases Sau3a and Taqi. Gene. 1980 Dec;12(3-4):267–275. doi: 10.1016/0378-1119(80)90109-2. [DOI] [PubMed] [Google Scholar]
  16. Tobler H., Smith K. D., Ursprung H. Molecular aspects of chromatin elimination in Ascaris lumbricoides. Dev Biol. 1972 Feb;27(2):190–203. doi: 10.1016/0012-1606(72)90097-8. [DOI] [PubMed] [Google Scholar]

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