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. 1982 Aug 25;10(16):4831–4843. doi: 10.1093/nar/10.16.4831

Blotting of RNA onto ion exchange paper allowing subsequent characterization by in situ translation in addition to blot hybridization.

C J Saris, H J Franssen, J H Heuyerjans, J van Eenbergen, H P Bloemers
PMCID: PMC320821  PMID: 7134000

Abstract

We present the preparation of an ion exchange paper which can be used as a solid carrier in the transfer of RNA from gels. In addition to detection by blot hybridization to specific probes, transferred RNA can be characterized by cell-free translation in situ. Preparation of the paper is simple, inexpensive and reproducible. Examples of applications are shown and possible other applications are discussed.

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Selected References

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