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. 1982 Aug 25;10(16):4883–4890. doi: 10.1093/nar/10.16.4883

The expression of biologically active cholera toxin in Escherichia coli.

M L Gennaro, P J Greenaway, D A Broadbent
PMCID: PMC320838  PMID: 6290992

Abstract

Chromosomal DNA from Vibrio cholerae El Tor strain 1621 was digested with Hind III and the products fractionated by centrifugation through a sucrose gradient. A 15kb fragment containing the toxin gene of V. cholerae was identified by its homology with the heat labile toxin (LT) gene of toxigenic E. coli. This fragment was cloned in E. coli using pAT153 and subsequently characterised by digestion with different restriction endonucleases. Sequences homologous to the LT gene were identified by hybridisation and then sub-cloned using either pAT153 or pACYC184. Expression of the cloned CT gene in E. coli was detected using both cell culture and ELISA assays. One recombinant plasmid coded for the synthesis of an immunologically active but biologically inactive derivative of CT.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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