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. 1982 Aug 25;10(16):5059–5072. doi: 10.1093/nar/10.16.5059

Influence of nonhistone chromatin protein HMG-1 on the enzymatic digestion of purified DNA.

K Shastri, P J Isackson, J L Fishback, M D Land, G R Reeck
PMCID: PMC320851  PMID: 6291002

Abstract

The effect of chicken erythrocyte High Mobility Group protein 1 (HMG-1) on the enzymatic hydrolysis of purified double-stranded and single-stranded bacteriophage lambda DNA was studied. HMG-1 was found to inhibit the digestion of single- and double-stranded DNA by S1 nuclease and DNase I, respectively. HMG-I increased the rate of hydrolysis of double-stranded DNA by micrococcal nuclease, particularly at low HMG-1/DNA ratios, and had little effect on the hydrolysis of single-stranded DNA by micrococcal nucleases, even at high HMG-1 DNA ratios. We also present a semi-quantitative estimate that HMG-1 and HMG-2 occur in chromatin from rapidly dividing, cultured rat hepatoma cells at about 8 times the level that they occur in adult rat liver chromatin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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