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. 1982 Sep 11;10(17):5223–5238. doi: 10.1093/nar/10.17.5223

A yeast transcription system for the 5S rRNA gene.

H van Keulen, D Y Thomas
PMCID: PMC320867  PMID: 7145700

Abstract

A cell-free extract of yeast nuclei that can specifically transcribe cloned yeast 5S rRNA genes has been developed. Optima for transcription of 5S rDNA were determined and conditions of extract preparation leading to reproducible activities and specificities established. The major in vitro product has the same size and oligonucleotide composition as in vivo 5S rRNA. The in vitro transcription extract does not transcribe yeast tRNA genes. The extract does increase the transcription of tRNA genes packaged in chromatin.

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Selected References

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