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. 2011 May 23;30(44):4498–4508. doi: 10.1038/onc.2011.154

Figure 7.

Figure 7

Observing the effect of interfering PAK1, an effector of BDKRB2 in IRX1-overexpressed cancer cells. (a) Overexpression of IRX1 downregulated PAK1 expressing level, whereas knockdown of PAK1 level did not alter the IRX1 level significantly. Overexpression of IRX1 combined with siRNA-PAK1 also downregulated PAK1 expressing level significantly by western blot. (b) The cell proliferation activity was suppressed significantly in 7901/IRX1 or 7901/siRNA-PAK1 groups, compared with controls (*P<0.001). The cell proliferation activity in 7901/IRX1/siRNA-PAK1 co-transfected group was nearly the same as that observed in 7901/IRX1 alone group (P=0.061). (c) By transwell assays, the migrated cell numbers (top) were significantly reduced in 7901/IRX1 group (77.33±4.25) or 7901/siRNA-PAK1 group (84±1), compared with control (206.66±8.5, *P<0.001). Whereas in 7901/IRX1/siRNA-PAK1 co-transfected group, the migrated cell numbers were nearly the same as that observed in 7901/IRX1 alone group (70.66±1.52 vs 77.33±4.25, P=0.202). The invasive cell numbers (down) were significantly decreased in 7901/IRX1 group (79.66±5.03) or 7901/siRNA-PAK1 group (71±6), compared with control (178±4.58, *P<0.001). Whereas in 7901/IRX1/siRNA-PAK1 co-transfected group, the invasive cell numbers were nearly the same as that observed in 7901/siRNA-PAK1 alone group 69±5.57 vs 71±6, *P=0.113). The data represent the mean±s.d. of three independent experiments.