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. 1982 Sep 25;10(18):5429–5445. doi: 10.1093/nar/10.18.5429

Accurate in vitro splicing of human beta-globin RNA.

R Kole, S M Weissman
PMCID: PMC320887  PMID: 6292841

Abstract

Human beta-globin RNA transcribed from an exogenous DNA template is spliced in vitro by concentrated whole cell extracts from HeLa cells. Using the primer extension technique, we have shown that the small intervening sequence is spliced accurately and that the sequence of the product across the splice junction is identical to that of beta-globin mRNA prepared from human reticulocytes. The efficiency of the splicing reaction is low. The RNA transcript containing both introns and terminated upstream from the polyadenylation site is spliced most efficiently. The transcript which is terminated downstream from the polyadenylation site is not spliced at all. Thalassemic beta-globin RNA which carries an extra splice site in the small intron is also spliced, albeit with a low yield.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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