Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1982 Sep 25;10(18):5637–5647. doi: 10.1093/nar/10.18.5637

RNA polymerase: linear competitive inhibition by bis-(3' to 5')-cyclic dinucleotides,NpNp.

C Y Hsu, D Dennis
PMCID: PMC320912  PMID: 7145712

Abstract

We have investigated the possible role of the bis-(3' to 5')-cyclic dinucleotides UpUp and ApUp as kinetic inhibitors of the DNA dependent RNA polymerase enzyme of E. coli, using T7 delta D111 deletion mutant DNA and several synthetic DNA polymers as templates. We have established that UpUp is a linear competitive inhibitor of the initiation phase of the polymerization (Ki = 28 microM using T7 delta D111 DNA as a template), but that it has no effect when added during the elongation phase. The compound ApUp is an inhibitor of the reaction only when poly(dA-T).poly(dA-T) is used as a template, and UpUp is an inhibitor of the reaction when poly(dA).poly(dT) was employed as the DNA template.

Full text

PDF
5637

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carpousis A. J., Gralla J. D. Cycling of ribonucleic acid polymerase to produce oligonucleotides during initiation in vitro at the lac UV5 promoter. Biochemistry. 1980 Jul 8;19(14):3245–3253. doi: 10.1021/bi00555a023. [DOI] [PubMed] [Google Scholar]
  2. Dennis D., Sylvester J. E. RNA polymerase: a model for rotational translocation. FEBS Lett. 1981 Feb 23;124(2):135–139. doi: 10.1016/0014-5793(81)80121-4. [DOI] [PubMed] [Google Scholar]
  3. Grachev M. A., Zaychikov E. F. Initiation by Escherichia coli RNA-polymerase: transformation of abortive to productive complex. FEBS Lett. 1980 Jun 16;115(1):23–26. doi: 10.1016/0014-5793(80)80718-6. [DOI] [PubMed] [Google Scholar]
  4. Hansen U. M., McClure W. R. Role of the sigma subunit of Escherichia coli RNA polymerase in initiation. II. Release of sigma from ternary complexes. J Biol Chem. 1980 Oct 25;255(20):9564–9570. [PubMed] [Google Scholar]
  5. Kinsella L., Hsu C. Y., Schulz W., Dennis D. RNA polymerase: correlation between transcript length, abortive product synthesis, and formation of a stable ternary complex. Biochemistry. 1982 May 25;21(11):2719–2723. doi: 10.1021/bi00540a022. [DOI] [PubMed] [Google Scholar]
  6. McClure W. R., Cech C. L. On the mechanism of rifampicin inhibition of RNA synthesis. J Biol Chem. 1978 Dec 25;253(24):8949–8956. [PubMed] [Google Scholar]
  7. Shimamoto N., Wu F. Y., Wu C. W. Mechanism of ribonucleic acid chain initiation. Molecular pulse-labeling study of ribonucleic acid syntheses on T7 deoxyribonucleic acid template. Biochemistry. 1981 Aug 4;20(16):4745–4755. doi: 10.1021/bi00519a034. [DOI] [PubMed] [Google Scholar]
  8. Smagowicz W. J., Scheit K. H. Primed abortive initiation of RNA synthesis by E. coli RNA polymerase on T7 DNA. Steady state kinetic studies. Nucleic Acids Res. 1978 Jun;5(6):1919–1932. doi: 10.1093/nar/5.6.1919. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Zillig W., Zechel K., Halbwachs H. J. A new method of large scale preparation of highly purified DNA-dependent RNA-polymerase from E. coli. Hoppe Seylers Z Physiol Chem. 1970 Feb;351(2):221–224. doi: 10.1515/bchm2.1970.351.1.221. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES