Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1982 Sep 25;10(18):5711–5716. doi: 10.1093/nar/10.18.5711

5S RNA sequence from the Philosamia silkworm: evidence for variable evolutionary rates in insect 5S RNA.

G Xian-Rong, K Nicoghosian, R J Cedergren
PMCID: PMC320918  PMID: 7145713

Abstract

The primary structure of 5S RNA isolated from the posterior silkgland of Philosamia cynthia ricini was determined using three in vitro labelling techniques. The derived sequence consists of 119 nucleotides and can be folded into the secondary structure model proposed for eukaryotic 5S RNAs. This 5S RNA differs from the Bombyx mori molecule in 9 positions and from the Drosophila melanogaster sequence in 14 positions. The comparison of evolutionary rates in insect 5S RNA with inferred rates in other eukaryotic phyla leads to the conclusion that 5S RNA evolution is not constant in different eukaryotic branches, a condition which must be taken into account in phylogenetic tree constructions.

Full text

PDF
5711

Images in this article

5713Image
on p.5713

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benhamou J., Jordan B. R. Nucleotide sequence of Drosophila melanogaster 5S RNA: evidence for a general 5S RNA model. FEBS Lett. 1976 Feb 15;62(2):146–149. doi: 10.1016/0014-5793(76)80039-7. [DOI] [PubMed] [Google Scholar]
  2. Canaday J., Guillemaut P., Gloeckler R., Weil J. H. The nucleotide sequence of spinach chloroplast tryptophan transfer RNA. Nucleic Acids Res. 1981 Jan 10;9(1):47–53. doi: 10.1093/nar/9.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cedergren R. J., Sankoff D., LaRue B., Grosjean H. The evolving tRNA molecule. CRC Crit Rev Biochem. 1981;11(1):35–104. doi: 10.3109/10409238109108699. [DOI] [PubMed] [Google Scholar]
  4. Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hori H., Osawa S. Evolutionary change in 5S RNA secondary structure and a phylogenic tree of 54 5S RNA species. Proc Natl Acad Sci U S A. 1979 Jan;76(1):381–385. doi: 10.1073/pnas.76.1.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. MacKay R. M., Doolittle W. F. Nucleotide sequences of Acanthamoeba castellanii 5S and 5.8S ribosomal ribonucleic acids: phylogenetic and comparative structural analyses. Nucleic Acids Res. 1981 Jul 24;9(14):3321–3334. doi: 10.1093/nar/9.14.3321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Peattie D. A. Direct chemical method for sequencing RNA. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1760–1764. doi: 10.1073/pnas.76.4.1760. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sankoff D., Cedergren R. J., McKay W. A strategy for sequence phylogeny research. Nucleic Acids Res. 1982 Jan 11;10(1):421–431. doi: 10.1093/nar/10.1.421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Schwartz R. M., Dayhoff M. O. Origins of prokaryotes, eukaryotes, mitochondria, and chloroplasts. Science. 1978 Jan 27;199(4327):395–403. doi: 10.1126/science.202030. [DOI] [PubMed] [Google Scholar]
  10. Stanley J., Vassilenko S. A different approach to RNA sequencing. Nature. 1978 Jul 6;274(5666):87–89. doi: 10.1038/274087a0. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES