Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1982 Oct 11;10(19):5799–5808. doi: 10.1093/nar/10.19.5799

Genes for tRNALys5 from Drosophila melanogaster.

D DeFranco, K B Burke, S Hayashi, G M Tener, R C Miller Jr, D Söll
PMCID: PMC320931  PMID: 6292853

Abstract

The sequences of two cloned genes from Drosophila which hybridize with tRNALys5 are reported. One gene, in plasmid pDt39, has a sequence which corresponds to the sequence of tRNA. The other gene, in pDt59R, differs in three nucleotides pairs. Both plasmids are transcribed in vitro with extracts of Drosophila Kc cells to give full-sized tRNA precursors with four additional nucleotides at the 5'-end as well as truncated molecules containing 35 nucleotides. This premature termination occurs in a block of four T residues within the mature coding region. Sequences flanking the tRNA genes show little in common except for the blocks of five or more T-residues beyond the 3'-end of the gene. pDt39 hybridizes to 84AB on the polytene chromosomes of Drosophila and pDt59R hybridizes to 29A.

Full text

PDF
5799

Images in this article

5802Image
on p.5802
5802Image
on p.5802
5805Image
on p.5805

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Addison W. R., Astell C. R., Delaney A. D., Gillam I. C., Hayashi S., Miller R. C., Rajput B., Smith M., Taylor D. M., Tener G. M. The structures of genes hybridizing with tRNA4Val from Drosophila melanogaster. J Biol Chem. 1982 Jan 25;257(2):670–673. [PubMed] [Google Scholar]
  2. Bogenhagen D. F., Brown D. D. Nucleotide sequences in Xenopus 5S DNA required for transcription termination. Cell. 1981 Apr;24(1):261–270. doi: 10.1016/0092-8674(81)90522-5. [DOI] [PubMed] [Google Scholar]
  3. Ciliberto G., Castagnoli L., Melton D. A., Cortese R. Promoter of a eukaryotic tRNAPro gene is composed of three noncontiguous regions. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1195–1199. doi: 10.1073/pnas.79.4.1195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DeFranco D., Schmidt O., Söll D. Two control regions for eukaryotic tRNA gene transcription. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3365–3368. doi: 10.1073/pnas.77.6.3365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DeFranco D., Sharp S., Söll D. Identification of regulatory sequences contained in the 5'-flanking region of Drosophila lysine tRNA2 genes. J Biol Chem. 1981 Dec 10;256(23):12424–12429. [PubMed] [Google Scholar]
  6. Dingermann T., Sharp S., Appel B., DeFranco D., Mount S., Heiermann R., Pongs O., Söll D. Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract. Nucleic Acids Res. 1981 Aug 25;9(16):3907–3918. doi: 10.1093/nar/9.16.3907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dunn R., Delaney A. D., Gillam I. C., Hayashi S., Tener G. M., Grigliatti T., Misra V., Spurr M. G., Taylor D. M., Miller R. C., Jr Isolation and characterization of recombinant DNA plasmids carrying Drosophila tRNA genes. Gene. 1979 Nov;7(3-4):197–215. doi: 10.1016/0378-1119(79)90046-5. [DOI] [PubMed] [Google Scholar]
  8. Dunn R., Hayashi S., Gillam I. C., Delaney A. D., Tener G. M., Grigliatti T. A., Kaufman T. C., Suzuki D. T. Genes coding for valine transfer ribonucleic acid-3b in Drosophila melanogaster. J Mol Biol. 1979 Mar 5;128(3):277–287. doi: 10.1016/0022-2836(79)90088-3. [DOI] [PubMed] [Google Scholar]
  9. Elder R. T., Szabo P., Uhlenbeck O. C. In situ hybridization of three transfer RNAs to the polytene chromosomes of Drosophila melanogaster. J Mol Biol. 1980 Sep 5;142(1):1–17. doi: 10.1016/0022-2836(80)90202-8. [DOI] [PubMed] [Google Scholar]
  10. Gergen J. P., Loewenberg J. Y., Wensink P. C. tRNA2Lys gene clusters in Drosophila. J Mol Biol. 1981 Apr 25;147(4):475–499. doi: 10.1016/0022-2836(81)90396-x. [DOI] [PubMed] [Google Scholar]
  11. Grigliatti T. A., White B. N., Tener G. M., Kaufman T. C., Suzuki D. T. The localization of transfer RNA5Lys genes in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3527–3531. doi: 10.1073/pnas.71.9.3527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hayashi S., Addison W. R., Gillam I. C., Grigliatti T. A., Tener G. M. Hybridization of tRNAs of Drosophila melanogaster to the region of the 5S RNA genes of the polytene chromosomes. Chromosoma. 1981;82(3):385–397. doi: 10.1007/BF00285764. [DOI] [PubMed] [Google Scholar]
  13. Hayashi S., Gillam I. C., Delaney A. D., Dunn R., Tener G. M., Grigliatti T. A., Suzuki D. T. Hybridization of tRNAs of Drosophila melanogaster to polytene chromosomes. Chromosoma. 1980;76(1):65–84. doi: 10.1007/BF00292227. [DOI] [PubMed] [Google Scholar]
  14. Hershey N. D., Davidson N. Two drosophila melanogaster tRNAGly genes are contained in a direct duplication at chromosomal locus 56F. Nucleic Acids Res. 1980 Nov 11;8(21):4899–4910. doi: 10.1093/nar/8.21.4899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hofstetter H., Kressman A., Birnstiel M. L. A split promoter for a eucaryotic tRNA gene. Cell. 1981 May;24(2):573–585. doi: 10.1016/0092-8674(81)90348-2. [DOI] [PubMed] [Google Scholar]
  16. Hosbach H. A., Silberklang M., McCarthy B. J. Evolution of a D. melanogaster glutamate tRNA gene cluster. Cell. 1980 Aug;21(1):169–178. doi: 10.1016/0092-8674(80)90124-5. [DOI] [PubMed] [Google Scholar]
  17. Hovemann B., Sharp S., Yamada H., Söll D. Analysis of a drosophila tRNA gene cluster. Cell. 1980 Apr;19(4):889–895. doi: 10.1016/0092-8674(80)90080-x. [DOI] [PubMed] [Google Scholar]
  18. Kaufman T. C., Shannon M. P., Shen M. W., Judd B. H. A revision of the cytology and ontogeny of several deficiencies in the 3A1-3C6 region of the X chromosome of Drosophila melanogaster. Genetics. 1975 Feb;79(2):265–282. doi: 10.1093/genetics/79.2.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Korn L. J., Brown D. D. Nucleotide sequence of Xenopus borealis oocyte 5S DNA: comparison of sequences that flank several related eucaryotic genes. Cell. 1978 Dec;15(4):1145–1156. doi: 10.1016/0092-8674(78)90042-9. [DOI] [PubMed] [Google Scholar]
  20. Koski R. A., Clarkson S. G., Kurjan J., Hall B. D., Smith M. Mutations of the yeast SUP4 tRNATyr locus: transcription of the mutant genes in vitro. Cell. 1980 Nov;22(2 Pt 2):415–425. doi: 10.1016/0092-8674(80)90352-9. [DOI] [PubMed] [Google Scholar]
  21. Kubli E., Schmidt T. The localization of tRNA4Glu genes from Drosophila melanogaster by "in situ" hybridization. Nucleic Acids Res. 1978 May;5(5):1465–1478. doi: 10.1093/nar/5.5.1465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ritossa F. M., Atwood K. C., Spiegelman S. On the redundancy of DNA complementary to amino acid tranfer RNA and its absence from the nucleolar organizer region of Drosophila melanogaster. Genetics. 1966 Aug;54(2):663–676. doi: 10.1093/genetics/54.2.663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Robinson R. R., Davidson N. Analysis of a drosophila tRNA gene cluster: two tRNALeu genes contain intervening sequences. Cell. 1981 Jan;23(1):251–259. doi: 10.1016/0092-8674(81)90289-0. [DOI] [PubMed] [Google Scholar]
  24. Sanger F., Coulson A. R. The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978 Mar 1;87(1):107–110. doi: 10.1016/0014-5793(78)80145-8. [DOI] [PubMed] [Google Scholar]
  25. Schmidt T., Egg A. H., Kubli E. The localization of tRNAAsp2 genes from Drosophila melanogaster by "in situ" hybridization. Mol Gen Genet. 1978 Sep 8;164(3):249–254. doi: 10.1007/BF00333153. [DOI] [PubMed] [Google Scholar]
  26. Sharp S., DeFranco D., Dingermann T., Farrell P., Söll D. Internal control regions for transcription of eukaryotic tRNA genes. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6657–6661. doi: 10.1073/pnas.78.11.6657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sharp S., DeFranco D., Silberklang M., Hosbach H. A., Schmidt T., Kubli E., Gergen J. P., Wensink P. C., Söll D. The initiator tRNA genes of Drosophila melanogaster: evidence for a tRNA pseudogene. Nucleic Acids Res. 1981 Nov 25;9(22):5867–5882. doi: 10.1093/nar/9.22.5867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Silverman S., Schmidt O., Söll D., Hovemann B. The nucleotide sequence of a cloned Drosophila arginine tRNA gene and its in vitro transcription in Xenopus germinal vesicle extracts. J Biol Chem. 1979 Oct 25;254(20):10290–10294. [PubMed] [Google Scholar]
  29. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  30. Steffensen D. M., Wimber D. E. Localization of tRNA genes in the salivary chromosomes of Drosophila by RNA:DNA hybridization. Genetics. 1971 Oct;69(2):163–178. doi: 10.1093/genetics/69.2.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Tartof K. D., Perry R. P. The 5 s RNA genes of Drosophila melanogaster. J Mol Biol. 1970 Jul 28;51(2):171–183. doi: 10.1016/0022-2836(70)90135-x. [DOI] [PubMed] [Google Scholar]
  32. Weber L., Berger E. Base sequence complexity of the stable RNA species of Drosophila melanogaster. Biochemistry. 1976 Dec 14;15(25):5511–5519. doi: 10.1021/bi00670a015. [DOI] [PubMed] [Google Scholar]
  33. White B. N., Tener G. M., Holden J., Suzuki D. T. Analysis of tRNAs during the development of Drosophila. Dev Biol. 1973 Jul;33(1):185–195. doi: 10.1016/0012-1606(73)90173-5. [DOI] [PubMed] [Google Scholar]
  34. Yen P. H., Davidson N. The gross anatomy of a tRNA gene cluster at region 42A of the D. melanogaster chromosome. Cell. 1980 Nov;22(1 Pt 1):137–148. doi: 10.1016/0092-8674(80)90162-2. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES