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. 1982 Oct 11;10(19):5925–5934. doi: 10.1093/nar/10.19.5925

Cloning and characterization of five overlapping cDNAs specific for the human pro alpha 1(I) collagen chain.

M L Chu, J C Myers, M P Bernard, J F Ding, F Ramirez
PMCID: PMC320940  PMID: 6183642

Abstract

We report the cloning of five overlapping cDNAs bearing sequences specific for the human pro alpha 1(I) collagen chain. Poly-A RNA enriched for collagen sequences was purified from normal human fibroblasts and used as template to synthesize double stranded cDNA. The cDNA was inserted into the Eco RI site of pBR 322 by blunt-ending and dG:dC tailing. The clones were screened by colony hybridization using the original RNA population and the resulting five positive clones subjected to restriction endonuclease mapping analysis and DNA sequencing. These overlapping clones cover from residue 247 in the alpha chain to part of the 3' end untranslated region of the pro alpha 1(I) mRNA for a total of 3400 nucleotides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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