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. 1982 Oct 11;10(19):5935–5948. doi: 10.1093/nar/10.19.5935

IS3 can function as a mobile promoter in E. coli.

D Charlier, J Piette, N Glansdorff
PMCID: PMC320941  PMID: 6292860

Abstract

We had shown previously (3) that the E. coli argE gene could be turned-on by an IS3 element inserted in orientation II near the 5' end of the gene. Here we show that this effect is due to the presence of an outward promoter located on IS3. The exact site of insertion of IS3 was determined by DNA sequencing. Using the S1 nuclease mapping technique with in vivo transcribed RNA we located the promoter responsible for argE transcription on IS3 itself outside the region involved in the inverted repeats of this element. IS3 may therefore be considered as a mobile promoter.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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