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. 1982 Oct 11;10(19):5967–5978. doi: 10.1093/nar/10.19.5967

Comparative DNA analysis of three South American marsupials.

A Heguy, H Musto, R Wettstein
PMCID: PMC320943  PMID: 6292862

Abstract

Published information on marsupials DNA is limited to a group of species belonging to only one genus. No previous reports have been written on South American species. In this paper we characterize the DNA of three out of the four marsupials found in Uruguay. Analytical and preparative ultracentrifugations in neutral CsCl gradients, including four intercalating agents and in Cs2SO4 gradients in presence of increasing amounts of Hg++ ion did not allow us to separate any satellite fraction. The buoyant density of the unique peak measured in CsCl gradients was in every case 1.697 g/cc with a G-C content of 37.7%. Digestion of total DNA with 11 restriction endonucleases produced a different pattern of bands for the three species, although some possible homologies could be established. Hybridization with 32P-rRNA of Southern blots of the gels containing digested DNAs demonstrated that the repeated sequences evidenced do not correspond to the ribosomal cistrons.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Angerer R. C., Davidson E. H., Britten R. J. Single copy DNA and structural gene sequence relationships among four sea urchin species. Chromosoma. 1976 Jul 8;56(3):213–226. doi: 10.1007/BF00293186. [DOI] [PubMed] [Google Scholar]
  2. Brown S. D., Dover G. A. Conservation of sequences in related genomes of Apodemus: constraints on the maintenance of satellite DNA sequences. Nucleic Acids Res. 1979 Jun 11;6(7):2423–2434. doi: 10.1093/nar/6.7.2423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dennis E. S., Dunsmuir P., Peacock W. J. Segmental amplification in a satellite DNA: restriction enzyme analysis of the major satellite of Macropus rufogriseus. Chromosoma. 1980;79(2):179–198. doi: 10.1007/BF01175184. [DOI] [PubMed] [Google Scholar]
  4. Duhamel-Maestracci N., Simard R., Harbers K., Spencer J. H. Localization of satellite DNAs in the chromosomes of the guinea pig. Chromosoma. 1979 Oct 2;75(1):63–74. doi: 10.1007/BF00330625. [DOI] [PubMed] [Google Scholar]
  5. Dunsmuir P. Satellite DNA in the kangaroo Macropus rufogriseus. Chromosoma. 1976 Jun 30;56(2):111–125. doi: 10.1007/BF00293111. [DOI] [PubMed] [Google Scholar]
  6. Fry K., Salser W. Nucleotide sequences of HS-alpha satellite DNA from kangaroo rat Dipodomys ordii and characterization of similar sequences in other rodents. Cell. 1977 Dec;12(4):1069–1084. doi: 10.1016/0092-8674(77)90170-2. [DOI] [PubMed] [Google Scholar]
  7. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  8. Hatch F. T., Bodner A. J., Mazrimas J. A., Moore D. H. Satellite DNA and cytogenetic evolution. DNA quantity, satellite DNA and karyotypic variations in kangaroo rats (genus Dipodomys). Chromosoma. 1976 Oct 28;58(2):155–168. doi: 10.1007/BF00701356. [DOI] [PubMed] [Google Scholar]
  9. Long E. O., Dawid I. B. Repeated genes in eukaryotes. Annu Rev Biochem. 1980;49:727–764. doi: 10.1146/annurev.bi.49.070180.003455. [DOI] [PubMed] [Google Scholar]
  10. Maizels N. Dictyostelium 17S, 25S, and 5S rDNAs lie within a 38,000 base pair repeated unit. Cell. 1976 Nov;9(3):431–438. doi: 10.1016/0092-8674(76)90088-x. [DOI] [PubMed] [Google Scholar]
  11. Mazrimas J. A., Hatch F. T. Similarity of satilite DNA properties in the order Rodentia. Nucleic Acids Res. 1977 Sep;4(9):3215–3227. doi: 10.1093/nar/4.9.3215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Miklos G. L., John B. Heterochromatin and satellite DNA in man: properties and prospects. Am J Hum Genet. 1979 May;31(3):264–280. [PMC free article] [PubMed] [Google Scholar]
  13. Miklos G. L., Willcocks D. A., Baverstock P. R. Restriction endonuclease and molecular analyses of three rat genomes with special reference to chromosome rearrangement and speciation problems. Chromosoma. 1980;76(3):339–363. doi: 10.1007/BF00327271. [DOI] [PubMed] [Google Scholar]
  14. Pardue M. L., Gall J. G. Chromosomal localization of mouse satellite DNA. Science. 1970 Jun 12;168(3937):1356–1358. doi: 10.1126/science.168.3937.1356. [DOI] [PubMed] [Google Scholar]
  15. Peacock W. J., Dennis E. S., Elizur A., Calaby J. H. Repeated DNA sequences and kangaroo phylogeny. Aust J Biol Sci. 1981;34(3):325–340. doi: 10.1071/bi9810325. [DOI] [PubMed] [Google Scholar]
  16. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  17. Venolia L., Peacock W. J. A highly repeated DNA from the genome of the wallaroo (Macropus robustus robustus). Aust J Biol Sci. 1981;34(1):97–113. [PubMed] [Google Scholar]

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